PMID- 12852450
OWN - NLM
STAT- MEDLINE
DCOM- 20040105
LR  - 20190815
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 32
IP  - 1
DP  - 2003 Apr 24
TI  - Optimised procedures for the reversed-phase liquid chromatographic analysis of 
      formulations containing tricyclic antidepressants.
PG  - 71-84
AB  - The chromatographic behaviour (retention, selectivity, peak shape and resolution) 
      of seven tricyclic antidepressants (TCAs), amitryptiline, clomipramine, doxepin, 
      imipramine, maprotiline, nortryptiline and trimipramine, was examined. 
      Conventional unendcapped Cs and C18 columns and an endcapped XTerra MS C18 column 
      recommended for the analysis of basic compounds were used together with 
      acetonitrile-water and micellar sodium dodecylsulfate (SDS)-pentanol mobile 
      phases. The two best combinations were XTerra C18/acetonitrile, which yielded the 
      largest efficiencies and resolution, and C8/SDS-pentanol, which eliminated the 
      peak tails that were still observed with the XTerra C18 column. Both the systems 
      were used to develop simple chromatographic procedures for the control of TCAs in 
      pharmaceutical formulations using UV detection. The selected mobile phase 
      compositions were 35% (v/v) acetonitrile (XTerra C18 column) and 0.075 M SDS-6% 
      (v/v) pentanol (C8 column), both at pH 3. Satisfactory recoveries were achieved 
      in both cases, with intra- and inter-day relative standard deviations (RSDs) 
      always below 0.6 and 2.0%, respectively. The preparation of the samples was 
      simple in both modes, since a previous extraction of the drugs was not needed. 
      The micellar mode has, however, the advantage of using a smaller amount of 
      organic solvent, which is retained in the micellar SDS solution. The C8 column is 
      also less expensive.
FAU - Ruiz-Angel, M J
AU  - Ruiz-Angel MJ
AD  - Facultat de Quimica, Departament de Quimica Analitica, Universitat de Valencia, 
      Dr. Moliner 50, Burjassot, Valencia 46100, Spain.
FAU - Carda-Broch, S
AU  - Carda-Broch S
FAU - Simo-Alfonso, E F
AU  - Simo-Alfonso EF
FAU - Garcia-Alvarez-Coque, M C
AU  - Garcia-Alvarez-Coque MC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Acetonitriles)
RN  - 0 (Antidepressive Agents, Tricyclic)
RN  - 0 (Capsules)
RN  - 0 (Solvents)
RN  - 0 (Tablets)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetonitriles/chemistry
MH  - Antidepressive Agents, Tricyclic/*analysis/chemistry
MH  - Capsules
MH  - Chromatography, High Pressure Liquid/methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solvents/chemistry
MH  - Tablets
EDAT- 2003/07/11 05:00
MHDA- 2004/01/06 05:00
CRDT- 2003/07/11 05:00
PHST- 2003/07/11 05:00 [pubmed]
PHST- 2004/01/06 05:00 [medline]
PHST- 2003/07/11 05:00 [entrez]
AID - S0731-7085(03)00048-7 [pii]
AID - 10.1016/s0731-7085(03)00048-7 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2003 Apr 24;32(1):71-84. doi: 10.1016/s0731-7085(03)00048-7.