PMID- 12853156
OWN - NLM
STAT- MEDLINE
DCOM- 20040305
LR  - 20190827
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 88
IP  - 1
DP  - 2003 Jul 3
TI  - Altered function of murine mast cells in response to lipopolysaccharide and 
      peptidoglycan.
PG  - 21-6
AB  - Toll-like receptors (TLRs) recognize and signal the presence of bacterial 
      components such as lipopolysaccharide (LPS) and peptidoglycan (PG) as a part of 
      innate immunity. Our previous studies revealed that mast cells function as 
      effector cells in the protection of mice against lethal enterobacterial 
      infections. In this study, we examined both the gene expression of molecules 
      involved in TLR signaling and the effects of LPS and PG in bone marrow-derived 
      cultured mast cells (BMCMCs). The mRNA expression of TLR2, TLR4 and TLR6 was 
      detected in BMCMCs. CD14, MD-2 and MyD88, which are also involved in TLR pathway, 
      were also expressed. Neither LPS nor PG affected degranulation in BMCMCs, but 
      release of tumor necrosis factor increased slightly in response to LPS and PG. 
      Both LPS and PG enhanced expression of pro-matrix metalloproteinase 9 (pro-MMP-9) 
      in a dose-dependent manner, and DNA fragmentation was induced by LPS, but not by 
      PG. These results suggest that mast cells are the targets of LPS and PG, and that 
      the functions of these molecules produced exclusively by bacteria partly overlap, 
      but are distinct.
FAU - Ikeda, Teruo
AU  - Ikeda T
AD  - Research Institute of Biosciences, Azabu University, 1-17-71 Fuchinobe, 
      Sagamihara, Kanagawa 229-8501, Japan. ikedat@azabu.u.ac.jp
FAU - Funaba, Masayuki
AU  - Funaba M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Peptidoglycan)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tlr6 protein, mouse)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptor 6)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Cell Degranulation
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - DNA Fragmentation
MH  - Gene Expression
MH  - Lipopolysaccharide Receptors/genetics/metabolism
MH  - Lipopolysaccharides/*immunology
MH  - Mast Cells/*physiology
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice
MH  - Peptidoglycan/*immunology
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptor 6
MH  - Toll-Like Receptors
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2003/07/11 05:00
MHDA- 2004/03/06 05:00
CRDT- 2003/07/11 05:00
PHST- 2003/07/11 05:00 [pubmed]
PHST- 2004/03/06 05:00 [medline]
PHST- 2003/07/11 05:00 [entrez]
AID - S0165247803000312 [pii]
AID - 10.1016/s0165-2478(03)00031-2 [doi]
PST - ppublish
SO  - Immunol Lett. 2003 Jul 3;88(1):21-6. doi: 10.1016/s0165-2478(03)00031-2.