PMID- 12855112
OWN - NLM
STAT- MEDLINE
DCOM- 20030731
LR  - 20191107
IS  - 0898-2104 (Print)
IS  - 0898-2104 (Linking)
VI  - 13
IP  - 2
DP  - 2003 May
TI  - The effect of shear on the desorption of liposomes adsorbed to bacterial 
      biofilms.
PG  - 187-97
AB  - With the aid of a flow cell assembly the desorption of cationic liposomes 
      prepared from mixtures of dipalmitoylphoshatidylcholine (DDPC), cholesterol, and 
      either dimethyldioctadecylammonium bromide (DDAB) or 
      3,beta[N-(N1,N-dimethylethylenediamine)-carbamoyl]cholesterol (DC-chol) from 
      immobilized biofilms of Staphylococcus aureus has been studied as a function of 
      shear stress by confocal microscopy. A shear stress theory has been adapted from 
      fluid mechanics of laminar flow between parallel plates and used to determine the 
      critical shear stress for liposome desorption. The critical shear stress for both 
      DDAB and DC-chol liposomes has been determined as a function of cationic lipid 
      content and hence surface charge as reflected in their zeta potentials. The 
      critical shear stress has been used to obtain the potential energy of 
      liposome-biofilm interaction which together with the electrostatic interaction 
      energy has enabled estimates of the London-Hamaker constants to be made. The 
      values of the London-Hamaker constants at small liposome-bacterial cell 
      separation were found to be independent of liposome composition.
FAU - Ahmed, Khalid
AU  - Ahmed K
AD  - School of Biological Sciences, University of Manchester, Manchester, UK.
FAU - Jones, Malcolm N
AU  - Jones MN
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Liposome Res
JT  - Journal of liposome research
JID - 9001952
RN  - 0 (Liposomes)
SB  - IM
MH  - Adsorption
MH  - *Biofilms
MH  - Liposomes/*chemistry
MH  - Microscopy, Confocal
MH  - *Rheology
MH  - Staphylococcus aureus
MH  - Static Electricity
EDAT- 2003/07/12 05:00
MHDA- 2003/08/02 05:00
CRDT- 2003/07/12 05:00
PHST- 2003/07/12 05:00 [pubmed]
PHST- 2003/08/02 05:00 [medline]
PHST- 2003/07/12 05:00 [entrez]
AID - 120020320 [pii]
AID - 10.1081/lpr-120020320 [doi]
PST - ppublish
SO  - J Liposome Res. 2003 May;13(2):187-97. doi: 10.1081/lpr-120020320.