PMID- 12857913
OWN - NLM
STAT- MEDLINE
DCOM- 20030819
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 77
IP  - 15
DP  - 2003 Aug
TI  - Equine infectious anemia virus utilizes host vesicular protein sorting machinery 
      during particle release.
PG  - 8440-7
AB  - A final step in retrovirus assembly, particle release from the cell, is modulated 
      by a small motif in the Gag protein known as a late domain. Recently, human 
      immunodeficiency virus type 1 (HIV-1) and Moloney murine leukemia virus (M-MuLV) 
      were shown to require components of the cellular vacuolar protein sorting (VPS) 
      machinery for efficient viral release. HIV-1 interacts with the VPS pathway via 
      an association of HIV-1 Gag with TSG101, a component of the cellular complexes 
      involved in VPS. Equine infectious anemia virus (EIAV) is unique among enveloped 
      viruses studied to date because it utilizes a novel motif, YPDL in Gag, as a late 
      domain. Our analysis of EIAV assembly demonstrates that EIAV Gag release is 
      blocked by inhibition of the VPS pathway. However, in contrast to HIV-1, EIAV Gag 
      release is insensitive to TSG101 depletion and EIAV particles do not contain 
      significant levels of TSG101. Finally, we demonstrate that fusing EIAV Gag 
      directly with another cellular component of the VPS machinery, VPS28, can restore 
      efficient release of an EIAV Gag late-domain mutant. These results provide 
      evidence that retroviruses can interact with the cellular VPS machinery in 
      several different ways to accomplish particle release.
FAU - Tanzi, Giancarlo O
AU  - Tanzi GO
AD  - Department of Microbiology, University of Pennsylvania School of Medicine, 
      Philadelphia, Pennsylvania 19104-6076, USA.
FAU - Piefer, Andrew J
AU  - Piefer AJ
FAU - Bates, Paul
AU  - Bates P
LA  - eng
GR  - T32 GM007229/GM/NIGMS NIH HHS/United States
GR  - T32 NS007180/NS/NINDS NIH HHS/United States
GR  - T32-GM07229/GM/NIGMS NIH HHS/United States
GR  - T32-NS07180/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
RN  - 0 (GTPase-Activating Proteins)
RN  - 0 (Gene Products, gag)
RN  - 0 (Transcription Factors)
RN  - 0 (Tsg101 protein)
RN  - 0 (VPS28 protein, human)
RN  - 0 (Vesicular Transport Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Endosomal Sorting Complexes Required for Transport
MH  - GTPase-Activating Proteins/genetics/*metabolism
MH  - Gene Products, gag/genetics/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Infectious Anemia Virus, Equine/*physiology
MH  - Microscopy, Fluorescence
MH  - Mutation
MH  - Protein Transport/*physiology
MH  - Transcription Factors/genetics/metabolism
MH  - Vesicular Transport Proteins
MH  - Virion/*metabolism
PMC - PMC165230
EDAT- 2003/07/15 05:00
MHDA- 2003/08/20 05:00
PMCR- 2003/08/01
CRDT- 2003/07/15 05:00
PHST- 2003/07/15 05:00 [pubmed]
PHST- 2003/08/20 05:00 [medline]
PHST- 2003/07/15 05:00 [entrez]
PHST- 2003/08/01 00:00 [pmc-release]
AID - 0134 [pii]
AID - 10.1128/jvi.77.15.8440-8447.2003 [doi]
PST - ppublish
SO  - J Virol. 2003 Aug;77(15):8440-7. doi: 10.1128/jvi.77.15.8440-8447.2003.