PMID- 12858017
OWN - NLM
STAT- MEDLINE
DCOM- 20040223
LR  - 20091119
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 35
IP  - 3
DP  - 2003 Jun 30
TI  - Role of sphingomyelin-MAPKs pathway in heat-induced apoptosis.
PG  - 181-8
AB  - The role of sphingomyelinase (SMase) activation and mitogen activated protein 
      kinases (MAPKs) activation in cellular apoptosis was investigated during the 
      hyperthermic treatment of HL-60 human leukemia cells. Treating the cells for 1 h 
      at 43(o)C caused more than 50% of cellular apoptosis within several hours. The 
      neutral-SMase activity in the cells treated for 1 h at 42(o)C was slightly 
      increased but decreased in the cells treated at 43(o)C or 44(o)C for the same 
      period whereas the acid SMase activity was slightly increased after heating the 
      cells at 42(o)C and 43(o)C and markedly increased at 44(o)C for 1 h. Treatment of 
      cells with inhibitors of SMase activation and ceramide formation significantly 
      reduced the heat-induced apoptosis. Three major families of mitogen-activated 
      protein kinases (MAPKs), i.e. ERK1/2, p38 and JNK, were activated by the 
      hyperthermic treatment of cells. Inhibition of ERK1/2 with PD98059 exerted little 
      effect on the heat-induced apoptosis and p38 inhibition with SB203580 slightly 
      lessened apoptosis whereas, inhibition of JNK with SP600125 markedly suppressed 
      the heat-induced apoptosis. These results indicate that heat-shock induced the 
      activation of SMase, particularly acid-SMase, thereby causing apoptosis and that 
      JNK played a pivotal role in heat-induced apoptosis in HL-60 leukemia cells.
FAU - Chung, Hyun Sook
AU  - Chung HS
AD  - Department of Microbiology, Inha University, Incheon, Korea.
FAU - Park, So Ra
AU  - Park SR
FAU - Choi, Eun Kyung
AU  - Choi EK
FAU - Park, Heon-Jin
AU  - Park HJ
FAU - Griffin, Robert J
AU  - Griffin RJ
FAU - Song, Chang W
AU  - Song CW
FAU - Park, HeonJoo
AU  - Park H
LA  - eng
GR  - CA 44114/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
SB  - IM
MH  - Apoptosis/*physiology
MH  - Enzyme Activation
MH  - HL-60 Cells
MH  - Hot Temperature
MH  - Humans
MH  - Mitogen-Activated Protein Kinase Kinases/*metabolism
MH  - Sphingomyelin Phosphodiesterase/*metabolism
EDAT- 2003/07/15 05:00
MHDA- 2004/02/24 05:00
CRDT- 2003/07/15 05:00
PHST- 2003/07/15 05:00 [pubmed]
PHST- 2004/02/24 05:00 [medline]
PHST- 2003/07/15 05:00 [entrez]
AID - 200306306 [pii]
AID - 10.1038/emm.2003.25 [doi]
PST - ppublish
SO  - Exp Mol Med. 2003 Jun 30;35(3):181-8. doi: 10.1038/emm.2003.25.