PMID- 12860031
OWN - NLM
STAT- MEDLINE
DCOM- 20040324
LR  - 20190922
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 792
IP  - 2
DP  - 2003 Jul 25
TI  - Confirmation of amphetamine, methamphetamine, MDA and MDMA in urine samples using 
      disk solid-phase extraction and gas chromatography-mass spectrometry after 
      immunoassay screening.
PG  - 241-7
AB  - A method using mixed phase disk solid-phase extraction (SPE) and gas 
      chromatography-mass spectrometry (GC-MS) was developed for confirmation of 
      amphetamine (AMP), methamphetamine (MET), 3,4-methylenedioxyamphetamine (MDA) and 
      3,4-methylenedioxymethamphetamine (MDMA) in urine samples after immunoassay 
      screening. Disk SPE provided hydrophobic (C(18)) and strong cation-exchange (SCX) 
      interactions. The analytes were retained on SCX functional groups in the disk and 
      eluted with ammoniated ethyl acetate after washed with methanol. Confirmation and 
      quantitation was exercised by selected ion monitoring using nikethamide as 
      chromatographic standard. Recoveries of the amphetamines were between 73.0 and 
      104.6% with RSDs in range of 2.1-6.4% (n=3). The limits of detection were 2 ng/ml 
      for AMP, MET and MDMA, and 4 ng/ml for MDA. Five real urine samples were tested 
      with the method after immunoassay screening, and the results were comparable to 
      those of traditional liquid-liquid extraction (LLE). The method was 
      solvent-saved, simple, rapid and reliable, and the extract was cleaner than that 
      of LLE.
FAU - Huang, Zengping
AU  - Huang Z
AD  - Forensic Sciences Institute, Fujian Provincial Department of Public Security, 
      Fuzhou 350003, China.
FAU - Zhang, Shaoyu
AU  - Zhang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 44RAL3456C (Methamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - CK833KGX7E (Amphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/*urine
MH  - Amphetamine/*urine
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Immunoassay/*methods
MH  - Methamphetamine/*urine
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*urine
EDAT- 2003/07/16 05:00
MHDA- 2004/03/25 05:00
CRDT- 2003/07/16 05:00
PHST- 2003/07/16 05:00 [pubmed]
PHST- 2004/03/25 05:00 [medline]
PHST- 2003/07/16 05:00 [entrez]
AID - S1570023203002691 [pii]
AID - 10.1016/s1570-0232(03)00269-1 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 25;792(2):241-7. doi: 
      10.1016/s1570-0232(03)00269-1.