PMID- 12860038
OWN - NLM
STAT- MEDLINE
DCOM- 20040324
LR  - 20191210
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 792
IP  - 2
DP  - 2003 Jul 25
TI  - Determination of phloroglucinol in human plasma by high-performance liquid 
      chromatography-mass spectrometry.
PG  - 307-12
AB  - A sensitive and selective liquid chromatographic method coupled with mass 
      spectrometry (LC-MS) was developed for the quantification of phloroglucinol in 
      human plasma. Resorcinol was used as internal standard, with plasma samples 
      extracted using ethyl acetate. A centrifuged upper layer was then evaporated and 
      reconstituted with mobile phase. The reconstituted samples were injected into a 
      C(18) XTerra MS column (2.1 x 100 mm) with 3.5-microm particle size. The 
      analytical column lasted for at least 500 injections. The mobile phase was 15% 
      acetonitrile (pH 3.0), with flow-rate at 200 microl/min. The mass spectrometer 
      was operated in negative ion mode with selective ion monitoring (SIM). 
      Phloroglucinol was detected without severe interferences from plasma matrix when 
      used negative ion mode. Phloroglucinol produced a parent molecule ([M-H](-)) at 
      m/z 125 in negative ion mode. Detection of phloroglucinol in human plasma was 
      accurate and precise, with quantification limit at 5 ng/ml. This method has been 
      successfully applied to a study of phloroglucinol in human specimens.
FAU - Kim, Hohyun
AU  - Kim H
AD  - Department of Pharmacokinetics, Seoul Medical Science Institute, Seoul Clinical 
      Laboratories, 7-14, Dongbinggo-dong, Yongsan-gu, Seoul 140-809, South Korea. 
      novakims@yahoo.com
FAU - Roh, Hyeongjin
AU  - Roh H
FAU - Lee, Hee Joo
AU  - Lee HJ
FAU - Chung, Soo Youn
AU  - Chung SY
FAU - Choi, Sun Ok
AU  - Choi SO
FAU - Lee, Kyung Ryul
AU  - Lee KR
FAU - Han, Sang Beom
AU  - Han SB
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - DHD7FFG6YS (Phloroglucinol)
SB  - IM
MH  - Adult
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Male
MH  - Phloroglucinol/*blood/pharmacokinetics
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2003/07/16 05:00
MHDA- 2004/03/25 05:00
CRDT- 2003/07/16 05:00
PHST- 2003/07/16 05:00 [pubmed]
PHST- 2004/03/25 05:00 [medline]
PHST- 2003/07/16 05:00 [entrez]
AID - S1570023203003167 [pii]
AID - 10.1016/s1570-0232(03)00316-7 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 25;792(2):307-12. doi: 
      10.1016/s1570-0232(03)00316-7.