PMID- 12861033
OWN - NLM
STAT- MEDLINE
DCOM- 20030812
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 83
IP  - 7
DP  - 2003 Jul
TI  - Involvement of macrophage chemotactic protein-1 and interleukin-1beta during 
      inflammatory but not basic fibroblast growth factor-dependent neovascularization 
      in the mouse cornea.
PG  - 927-38
AB  - Corneal neovascularization develops in several pathologic conditions, but its 
      underlying mechanisms remain elusive. We used a mouse inflammatory corneal model 
      (corneas cauterized with silver nitrate) and assessed the role of 
      monocyte/macrophage-attracting factors, macrophage chemotactic protein-1 (MCP-1), 
      and a proinflammatory cytokine, IL-1beta, on macrophage recruitment and 
      neovascularization. Both MCP-1, IL-1beta protein, and mRNA levels increased 
      markedly 12 hours after the chemical cauterization. In situ hybridization showed 
      that MCP-1 was located in corneal epithelial cells, and IL-1beta was located in 
      corneal epithelial cells and infiltrating inflammatory cells. In addition, double 
      staining of corneas with antibodies specific for monocytes/macrophages and 
      IL-1beta revealed that IL-1beta was found in infiltrating monocytes/macrophages 
      at Day 2 after cauterization. Both IL-1beta and MCP-1 induced neovascularization 
      in a rat cornea model, and the cauterization-induced corneal neovascularization 
      was partially inhibited by subconjunctival injection of anti-IL-1beta or 
      anti-MCP-1. Coadministration of two antibodies inhibited corneal 
      neovascularization slightly more than that by the administration of each. In 
      contrast, administration of the anti-MCP-1 or anti-IL-1beta showed minimal 
      inhibition of basic fibroblast growth factor-driven corneal neovascularization by 
      mouse cornea assay. Cauterized corneas treated with anti-MCP-1 antibody had 
      significantly fewer monocytes/macrophages than control. These results indicate 
      the existence of distinct monocyte/macrophage-involved angiogenic pathways in 
      mouse cornea, in which MCP-1 released from corneal epithelial cells attracts 
      monocytes/macrophages into the cornea, where they release IL-1beta leading to 
      inflammatory neovascularization. In addition, the IL-1beta and MCP-1 released 
      from the corneal epithelial cells may directly induce corneal neovascularization.
FAU - Yoshida, Shigeo
AU  - Yoshida S
AD  - Department of Ophthalmology, Kyushu University Graduate School of Medicine, 
      Fukuoka, Japan.
FAU - Yoshida, Ayako
AU  - Yoshida A
FAU - Matsui, Hironori
AU  - Matsui H
FAU - Takada, Yu-Ichiro
AU  - Takada Y
FAU - Ishibashi, Tatsuro
AU  - Ishibashi T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Interleukin-1)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (RNA, Messenger)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Antibodies, Blocking/pharmacology
MH  - Cell Movement/drug effects
MH  - Chemokine CCL4
MH  - Cornea/*blood supply/drug effects/metabolism/pathology
MH  - Disease Models, Animal
MH  - Drug Synergism
MH  - Epithelium, Corneal/metabolism/pathology
MH  - Female
MH  - Fibroblast Growth Factor 2/*physiology
MH  - In Situ Hybridization
MH  - Interleukin-1/genetics/immunology/*metabolism/pharmacology
MH  - Macrophage Inflammatory Proteins/genetics/immunology/*metabolism/pharmacology
MH  - Macrophages/drug effects/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neovascularization, Pathologic/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/07/16 05:00
MHDA- 2003/08/13 05:00
CRDT- 2003/07/16 05:00
PHST- 2003/07/16 05:00 [pubmed]
PHST- 2003/08/13 05:00 [medline]
PHST- 2003/07/16 05:00 [entrez]
AID - S0023-6837(22)03492-4 [pii]
AID - 10.1097/01.lab.0000075642.11787.83 [doi]
PST - ppublish
SO  - Lab Invest. 2003 Jul;83(7):927-38. doi: 10.1097/01.lab.0000075642.11787.83.