PMID- 12866723
OWN - NLM
STAT- MEDLINE
DCOM- 20030815
LR  - 20041117
IS  - 1230-6002 (Print)
IS  - 1230-6002 (Linking)
VI  - 54
IP  - 6
DP  - 2002 Nov-Dec
TI  - Some drug effects on the activity of erythrocyte hexokinase and glucose 
      6-phosphate dehydrogenase enzymes in vitro and in vivo.
PG  - 673-9
AB  - Effects of theophylline, lidocaine, cyclophosphamide, hyoscine N-butyl bromide, 
      tranexamic acid and cytarabine on hexokinase (HK) from human erythrocytes have 
      been investigated in vitro. In addition, in vivo effects of theophylline and 
      lidocaine were investigated in rats. HK was purified from human red blood cells 
      by DEAE-Sephadex A50 ion exchange chromatography. HK activity was measured 
      spectrophotometrically at 25 degrees C in a system coupled with glucose 
      6-phosphate dehydrogenase, according to Beutler's method at 340 nm. 
      Cyclophosphamide, hyoscine N-butyl bromide, tranexamic acid and cytarabine had no 
      effects on human erythrocyte HK activity in in vitro conditions. On the other 
      hand, human erythrocyte HK was inhibited by theophylline, but activated by 
      lidocaine. IC50 value for theophylline was 0.013 M. In the case of in vivo 
      studies, 6 mg kg(-1) of theophylline inhibited the rat HK activity by 43% at the 
      first 1.5 h (p < 0.001). A dose of 5 mg kg(-1) of lidocaine activated the rat HK 
      activity by 41% (p < 0.001), 22% (p < 0.001), and 11% (p < 0.05), at 1.5, 3 and 6 
      h, respectively.
FAU - Ciftci, Mehmet
AU  - Ciftci M
AD  - Ataturk University, Faculty of Sciences and Arts, Department of Chemistry and 
      Biotechnology Application and Research Center, 25240 Erzurum, Turkey. 
      ciftcim@atauni.edu.tr
FAU - Senyayla, Faruk
AU  - Senyayla F
FAU - Ozdemir, Hasan
AU  - Ozdemir H
FAU - Buyukokuroglu, Mehmet E
AU  - Buyukokuroglu ME
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Pol J Pharmacol
JT  - Polish journal of pharmacology
JID - 9313882
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
SB  - IM
MH  - Animals
MH  - Enzyme Activation/drug effects/physiology
MH  - Erythrocytes/*drug effects/*enzymology
MH  - Glucosephosphate Dehydrogenase/*blood
MH  - Hexokinase/*blood
MH  - Humans
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2003/07/18 05:00
MHDA- 2003/08/16 05:00
CRDT- 2003/07/18 05:00
PHST- 2003/07/18 05:00 [pubmed]
PHST- 2003/08/16 05:00 [medline]
PHST- 2003/07/18 05:00 [entrez]
PST - ppublish
SO  - Pol J Pharmacol. 2002 Nov-Dec;54(6):673-9.