PMID- 12866848
OWN - NLM
STAT- MEDLINE
DCOM- 20031023
LR  - 20131121
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 26
IP  - 2
DP  - 2003 Jun
TI  - Long-term population dynamics and in situ physiology in activated sludge systems 
      with enhanced biological phosphorus removal operated with and without nitrogen 
      removal.
PG  - 211-27
AB  - Quantitative fluorescence in situ hybridization (FISH) and the combination of 
      FISH with microautoradiography (MAR) were used in order to study the long-term 
      population dynamics (2.5 years) and the in situ physiology in two parallel 
      activated sludge pilot systems with enhanced biological phosphorus removal 
      (EBPR). The two systems received the same influent wastewater, but were 
      differently operated (with and without nitrogen removal, respectively). Both 
      systems showed a significant P removal that increased when different substrates 
      (phosphorus (P), acetate and glucose, respectively) were added to the influent 
      wastewater. Rhodocyclus-related bacteria were present in both systems in 
      significant numbers (ranging from 4 to 28%) throughout the whole period. This 
      supports the hypothesis that these bacteria occur in significant numbers in 
      different types of well-operating EBPR activated sludge processes. However, we 
      observed a lower correlation (< 0.5) for the amount of Rhodocyclus-related 
      bacteria to the P content in activated sludge than previous studies (> 0.9). The 
      Actinobacteria were the only additional group of bacteria which showed a similar 
      degree of correlation to the P content in activated sludge as the 
      Rhodocyclus-related bacteria--but only for the system without nitrogen removal. 
      Significant amounts (< or = 12%) of glycogen-accumulating bacteria (GAOs) were 
      detected in the system with nitrogen removal (but not in the other system), but 
      had no, in contrast to previous observations, apparent negative effect on the 
      overall EBPR performance. FISH-MAR indicated that a significant part of the 
      Betaproteobacteria (part of them identified as Rhodocyclus-related bacteria) as 
      well as the Actinobacteria were able to take up 33Pi, [3H]-acetate and 
      [3H]-glucose under anaerobic-aerobic conditions. The contribution of anoxic 33Pi 
      uptake under alternating anaerobic-anoxic conditions was significantly lower. 
      Interestingly, not all Rhodocyclus-related bacteria showed uptake of these three 
      radioactive substrates. This may be due to differences in metabolic state, 
      physiological potential or genotype, not detectable by the present probe set for 
      Rhodocyclus-related bacteria. Comparison of the 33Pi, [3H]-acetate and 
      [3H]-glucose uptake by activated sludge after different fixation and incubation 
      procedures showed that a part of the observed 33Pi uptake may have been caused by 
      a combination of a biological and chemical or biologically induced chemical P 
      adsorption.
FAU - Lee, Natuschka
AU  - Lee N
AD  - Lehrstuhl fur Mikrobiologie, TU Munchen, Freising, Germany. 
      leen@micro.biologie.tu-muenchen.de
FAU - Nielsen, Per H
AU  - Nielsen PH
FAU - Aspegren, Henrik
AU  - Aspegren H
FAU - Henze, Mogens
AU  - Henze M
FAU - Schleifer, Karl-Heinz
AU  - Schleifer KH
FAU - la Cour Jansen, Jes
AU  - la Cour Jansen J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (Phosphorus Radioisotopes)
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
RN  - 9005-79-2 (Glycogen)
RN  - IY9XDZ35W2 (Glucose)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Archaea/isolation & purification
MH  - Autoradiography
MH  - Bacteria/classification/*isolation & purification/metabolism
MH  - Ecosystem
MH  - Fungi/isolation & purification
MH  - Glucose/metabolism
MH  - Glycogen/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - *Nitrogen/metabolism
MH  - *Phosphorus/metabolism
MH  - Phosphorus Radioisotopes/metabolism
MH  - Pilot Projects
MH  - Sewage/*microbiology
MH  - Sweden
MH  - Time Factors
MH  - Waste Disposal, Fluid/*methods
EDAT- 2003/07/18 05:00
MHDA- 2003/10/24 05:00
CRDT- 2003/07/18 05:00
PHST- 2003/07/18 05:00 [pubmed]
PHST- 2003/10/24 05:00 [medline]
PHST- 2003/07/18 05:00 [entrez]
AID - S0723-2020(04)70182-4 [pii]
AID - 10.1078/072320203322346065 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2003 Jun;26(2):211-27. doi: 10.1078/072320203322346065.