PMID- 12866858
OWN - NLM
STAT- MEDLINE
DCOM- 20031023
LR  - 20101118
IS  - 0723-2020 (Print)
IS  - 0723-2020 (Linking)
VI  - 26
IP  - 2
DP  - 2003 Jun
TI  - Characterisation of the bacterial community associated with early stages of Great 
      Scallop (Pecten maximus), using denaturing gradient gel electrophoresis (DGGE).
PG  - 302-11
AB  - Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA was used 
      to characterise and compare bacterial communities associated with scallop larvae 
      (Pecten maximus), in different production units in a shellfish hatchery. Water 
      and larvae samples were collected from three different aquaculture systems; 
      stagnant, flow-through and a flow- through system with seawater treated with 
      ozone. Samples were also collected from different algal cultures, inlet tanks and 
      water pipes leading to the different aquaculture systems. Clear differences were 
      seen between the bacterial community associated with the larvae and in the water 
      from the different aquaculture systems. However, there were high similarities in 
      the community composition between different water samples and between larvae 
      samples collected at different time periods, indicating a high stability in the 
      bacterial communities. Fifty three percent of the sequences from these samples 
      were similar to 16S rRNA gene sequences of members of the gamma-subclass of the 
      Proteobacteria. The different algal cultures had different bacterial communities, 
      however 73 percent of the sequences were similar to 16S rRNA gene sequences of 
      members of the alpha-subclass of the Proteobacteria. Differences in the DGGE 
      profiles were also seen between the samples taken from the inlet tanks and water 
      pipes, indicating a change in the bacterial community composition as the water 
      passed through the pipes. To our knowledge this is the first study investigating 
      bacterial communities associated with Great Scallop larvae in different 
      aquaculture systems including noncultured components.
FAU - Sandaa, Ruth-Anne
AU  - Sandaa RA
AD  - Institute of Marine Research, Bergen, Norway. ruth.sandaa@im.uib.no
FAU - Magnesen, Thorolf
AU  - Magnesen T
FAU - Torkildsen, Lise
AU  - Torkildsen L
FAU - Bergh, Oivind
AU  - Bergh O
LA  - eng
SI  - GENBANK/AY117756
SI  - GENBANK/AY117757
SI  - GENBANK/AY117758
SI  - GENBANK/AY117759
SI  - GENBANK/AY117760
SI  - GENBANK/AY117761
SI  - GENBANK/AY117762
SI  - GENBANK/AY117763
SI  - GENBANK/AY117764
SI  - GENBANK/AY117765
SI  - GENBANK/AY117766
SI  - GENBANK/AY117767
SI  - GENBANK/AY117768
SI  - GENBANK/AY117769
SI  - GENBANK/AY117770
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (Bacterial Proteins)
SB  - IM
MH  - Alphaproteobacteria/chemistry/classification/genetics/*isolation & purification
MH  - Animals
MH  - Aquaculture
MH  - Bacterial Proteins/analysis
MH  - Bacterial Typing Techniques
MH  - Ecosystem
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Eukaryota/microbiology
MH  - Gammaproteobacteria/chemistry/classification/genetics/*isolation & purification
MH  - Larva
MH  - Molecular Sequence Data
MH  - Mollusca/growth & development/*microbiology
MH  - Protein Denaturation
MH  - Ribotyping
EDAT- 2003/07/18 05:00
MHDA- 2003/10/24 05:00
CRDT- 2003/07/18 05:00
PHST- 2003/07/18 05:00 [pubmed]
PHST- 2003/10/24 05:00 [medline]
PHST- 2003/07/18 05:00 [entrez]
AID - S0723-2020(04)70192-7 [pii]
AID - 10.1078/072320203322346164 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2003 Jun;26(2):302-11. doi: 10.1078/072320203322346164.