PMID- 12867724
OWN - NLM
STAT- MEDLINE
DCOM- 20030923
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 65
IP  - 6
DP  - 2003 Jun
TI  - Generation of canine dendritic cells from peripheral blood mononuclear cells.
PG  - 663-9
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells that are 
      expected to be therapeutic agents for tumor immunotherapy. In this study, we 
      generated DCs of sufficient number for DC-based immunotherapy from peripheral 
      blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of 
      phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like 
      projections were seen, and the number of these large cells with projections 
      increased on day 8. These cells were positive for esterase staining. They 
      expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended 
      to make contact with lymphocytes under culture conditions. We obtained about 2-5 
      x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 
      cells by overnight co-culturing. These cells generated from PBMC are possible 
      canine DCs and are applicable to clinical trials of DC-based whole tumor cell 
      immunotherapy in dogs.
FAU - Yoshida, Hiroko
AU  - Yoshida H
AD  - Department of Veterinary Surgery, Faculty of Agriculture, Gifu University, Gifu, 
      Japan.
FAU - Momoi, Yasuyuki
AU  - Momoi Y
FAU - Taga, Natsuko
AU  - Taga N
FAU - Ide, Kaori
AU  - Ide K
FAU - Yamazoe, Kazuaki
AU  - Yamazoe K
FAU - Iwasaki, Toshiroh
AU  - Iwasaki T
FAU - Kudo, Tadaaki
AU  - Kudo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antigens, CD)
RN  - 0 (Culture Media)
RN  - 0 (Phytohemagglutinins)
RN  - EC 3.1.- (Esterases)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cricetinae
MH  - Culture Media/chemistry
MH  - Dendritic Cells/*cytology/drug effects/immunology/metabolism
MH  - Dogs
MH  - Esterases/metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Leukocytes, Mononuclear/*cytology/drug effects
MH  - Phagocytosis
MH  - Phytohemagglutinins/pharmacology
EDAT- 2003/07/18 05:00
MHDA- 2003/09/25 05:00
CRDT- 2003/07/18 05:00
PHST- 2003/07/18 05:00 [pubmed]
PHST- 2003/09/25 05:00 [medline]
PHST- 2003/07/18 05:00 [entrez]
AID - 10.1292/jvms.65.663 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2003 Jun;65(6):663-9. doi: 10.1292/jvms.65.663.