PMID- 12869137
OWN - NLM
STAT- MEDLINE
DCOM- 20030820
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 58
IP  - 2
DP  - 2003 Aug
TI  - Antigen presentation by murine dendritic cells is nuclear factor-kappa B 
      dependent both in vitro and in vivo.
PG  - 165-72
AB  - Antigen presentation is a key rate-limiting step in the immune response. 
      Dendritic cells (DCs) have been reported to be the most potent antigen-presenting 
      cells for naive T cells, but little is known about the biochemical pathways that 
      regulate this function. We here demonstrate that mature murine DC can be infected 
      with adenovirus at high efficiency (>95%) and that an adenovirus transferring the 
      endogenous inhibitor IkappaBalpha blocks nuclear factor-kappa B (NF-kappaB) 
      function in murine DC. This result indicates that antigen presentation in the 
      mixed leucocyte reaction is NF-kappaB dependent, confirming data with human DC in 
      vitro. However, the importance of this finding depends on verifying that this is 
      true also in vivo. Using delayed type hypersensitivity with allogeneic cells, we 
      show that NF-kappaB inhibition had a marked immunosuppressive effect in vivo. 
      These results thus establish NF-kappaB as an effective target for blocking DC 
      antigen presentation and hence inhibiting T-cell-dependent immune responses. This 
      finding has potential implications for the development of therapeutic agents for 
      use in various pathological conditions of the immune system, including allergy 
      and autoimmunity, and also in transplantation.
FAU - Yoshimura, S
AU  - Yoshimura S
AD  - Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College 
      of Science, Technology and Medicine, 1 Aspenlea Road, Hammersmith, London W6 8LH, 
      UK.
FAU - Bondeson, J
AU  - Bondeson J
FAU - Brennan, F M
AU  - Brennan FM
FAU - Foxwell, B M J
AU  - Foxwell BM
FAU - Feldmann, M
AU  - Feldmann M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Cytokines)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Adenoviridae/immunology
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Blotting, Western
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*immunology
MH  - Electrophoretic Mobility Shift Assay
MH  - Flow Cytometry
MH  - Genetic Vectors/immunology
MH  - Hypersensitivity, Delayed/immunology
MH  - I-kappa B Proteins/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/antagonists & inhibitors/*immunology
EDAT- 2003/07/19 05:00
MHDA- 2003/08/21 05:00
CRDT- 2003/07/19 05:00
PHST- 2003/07/19 05:00 [pubmed]
PHST- 2003/08/21 05:00 [medline]
PHST- 2003/07/19 05:00 [entrez]
AID - 1246 [pii]
AID - 10.1046/j.1365-3083.2003.01246.x [doi]
PST - ppublish
SO  - Scand J Immunol. 2003 Aug;58(2):165-72. doi: 10.1046/j.1365-3083.2003.01246.x.