PMID- 12873385
OWN - NLM
STAT- MEDLINE
DCOM- 20030818
LR  - 20190823
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 39
IP  - 2
DP  - 2003 Jul 17
TI  - Selective regulation of neurite extension and synapse formation by the beta but 
      not the alpha isoform of CaMKII.
PG  - 283-97
AB  - Neurite extension and branching are important neuronal plasticity mechanisms that 
      can lead to the addition of synaptic contacts in developing neurons and changes 
      in the number of synapses in mature neurons. Here we show that 
      Ca2+/calmodulin-dependent protein kinase II (CaMKII) regulates movement, 
      extension, and branching of filopodia and fine dendrites as well as the number of 
      synapses in hippocampal neurons. Only CaMKIIbeta, which peaks in expression early 
      in development, but not CaMKIIalpha, has this morphogenic activity. A small 
      insert in CaMKIIbeta, which is absent in CaMKIIalpha, confers regulated F-actin 
      localization to the enzyme and enables selective upregulation of dendritic 
      motility. These results show that the two main neuronal CaMKII isoforms have 
      markedly different roles in neuronal plasticity, with CaMKIIalpha regulating 
      synaptic strength and CaMKIIbeta controlling the dendritic morphology and number 
      of synapses.
FAU - Fink, Charles C
AU  - Fink CC
AD  - Department of Molecular Pharmacology, Stanford University Medical School, 
      Stanford, CA 94305, USA.
FAU - Bayer, Karl-Ulrich
AU  - Bayer KU
FAU - Myers, Jason W
AU  - Myers JW
FAU - Ferrell, James E Jr
AU  - Ferrell JE Jr
FAU - Schulman, Howard
AU  - Schulman H
FAU - Meyer, Tobias
AU  - Meyer T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (Actins)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Benzylamines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoquinolines)
RN  - 0 (KN 92)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Sulfonamides)
RN  - 0 (postsynaptic density proteins)
RN  - 0 (yellow fluorescent protein, Bacteria)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 9654F8OVKE (lucifer yellow)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Bacterial Proteins/metabolism
MH  - *Benzylamines
MH  - Binding Sites
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/classification/genetics/*physiology
MH  - Cell Fractionation/methods
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation, Enzymologic
MH  - Green Fluorescent Proteins
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Isoquinolines/metabolism
MH  - Luminescent Proteins/metabolism
MH  - Microscopy, Confocal
MH  - Mutation
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurites/drug effects/*physiology
MH  - Protein Isoforms/genetics/*physiology
MH  - RNA Interference/physiology
MH  - Rats
MH  - *Sulfonamides
MH  - Synapses/drug effects/*physiology
MH  - Time Factors
MH  - Transfection
EDAT- 2003/07/23 05:00
MHDA- 2003/08/19 05:00
CRDT- 2003/07/23 05:00
PHST- 2003/07/23 05:00 [pubmed]
PHST- 2003/08/19 05:00 [medline]
PHST- 2003/07/23 05:00 [entrez]
AID - S0896627303004288 [pii]
AID - 10.1016/s0896-6273(03)00428-8 [doi]
PST - ppublish
SO  - Neuron. 2003 Jul 17;39(2):283-97. doi: 10.1016/s0896-6273(03)00428-8.