PMID- 12874224
OWN - NLM
STAT- MEDLINE
DCOM- 20031107
LR  - 20220224
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 171
IP  - 3
DP  - 2003 Aug 1
TI  - Complexes of HLA-G protein on the cell surface are important for leukocyte 
      Ig-like receptor-1 function.
PG  - 1343-51
AB  - The nonclassical class I MHC molecule HLA-G is selectively expressed on 
      extravillous cytotrophoblast cells at the maternal-fetal interface during 
      pregnancy. HLA-G can inhibit the killing mediated by NK cells via interaction 
      with the inhibitory NK cell receptor, leukocyte Ig-like receptor-1 (LIR-1). 
      Comparison of the sequence of the HLA-G molecule to other class I MHC proteins 
      revealed two unique cysteine residues located in positions 42 and 147. Mutating 
      these cysteine residues resulted in a dramatic decrease in LIR-1 Ig binding. 
      Accordingly, the mutated HLA-G transfectants were less effective in the 
      inhibition of NK killing and RBL/LIR-1 induced serotonin release. 
      Immunoprecipitation experiments demonstrated the involvement of the cysteine 
      residues in the formation of HLA-G protein oligomers on the cell surface. The 
      cysteine residue located at position 42 is shown to be critical for the 
      expression of such complexes. These oligomers, unique among the class I MHC 
      proteins, probably bind to LIR-1 with increased avidity, resulting in an enhanced 
      inhibitory function of LIR-1 and an impaired killing function of NK cells.
FAU - Gonen-Gross, Tsufit
AU  - Gonen-Gross T
AD  - Lautenberg Center for General and Tumor Immunology, Hebrew University-Hadassah 
      Medical School, Jerusalem, Israel.
FAU - Achdout, Hagit
AU  - Achdout H
FAU - Gazit, Roi
AU  - Gazit R
FAU - Hanna, Jacob
AU  - Hanna J
FAU - Mizrahi, Sa'ar
AU  - Mizrahi S
FAU - Markel, Gal
AU  - Markel G
FAU - Goldman-Wohl, Debra
AU  - Goldman-Wohl D
FAU - Yagel, Simcha
AU  - Yagel S
FAU - Horejsi, Vaclav
AU  - Horejsi V
FAU - Levy, Ofer
AU  - Levy O
FAU - Baniyash, Michal
AU  - Baniyash M
FAU - Mandelboim, Ofer
AU  - Mandelboim O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (LILRB1 protein, human)
RN  - 0 (Leukocyte Immunoglobulin-like Receptor B1)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, CD/metabolism/*physiology
MH  - Binding, Competitive/genetics/immunology
MH  - Cell Line, Transformed
MH  - Cysteine/genetics/physiology
MH  - Cytotoxicity, Immunologic/genetics
MH  - Decidua/cytology/immunology
MH  - Down-Regulation/genetics/immunology
MH  - Female
MH  - HLA Antigens/biosynthesis/genetics/metabolism/*physiology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/biosynthesis/genetics/metabolism/*physiology
MH  - Humans
MH  - Leukocyte Immunoglobulin-like Receptor B1
MH  - Macromolecular Substances
MH  - Membrane Glycoproteins/biosynthesis/genetics/metabolism/*physiology
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Protein Binding/genetics/immunology
MH  - Rats
MH  - Receptors, Immunologic/antagonists & inhibitors/metabolism/*physiology
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 2003/07/23 05:00
MHDA- 2003/11/08 05:00
CRDT- 2003/07/23 05:00
PHST- 2003/07/23 05:00 [pubmed]
PHST- 2003/11/08 05:00 [medline]
PHST- 2003/07/23 05:00 [entrez]
AID - 10.4049/jimmunol.171.3.1343 [doi]
PST - ppublish
SO  - J Immunol. 2003 Aug 1;171(3):1343-51. doi: 10.4049/jimmunol.171.3.1343.