PMID- 12874302
OWN - NLM
STAT- MEDLINE
DCOM- 20030826
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 71
IP  - 8
DP  - 2003 Aug
TI  - Coordinate cytokine gene expression in vivo following induction of tuberculous 
      pleurisy in guinea pigs.
PG  - 4271-7
AB  - Tuberculous pleurisy is a severe inflammatory response induced by Mycobacterium 
      tuberculosis organisms that have escaped from lung granulomata into the pleural 
      space during pulmonary infection. We have used the guinea pig model of 
      tuberculous pleurisy to examine several aspects of the immune response to this 
      antigen-specific inflammatory event. Pleurisy was induced by injection of 
      heat-killed M. tuberculosis H37Rv directly into the pleural space of guinea pigs 
      previously vaccinated with M. bovis BCG. Four animals were euthanized each day 
      over a period of 9 days. Fluid in the pleural cavity was analyzed for 
      transforming growth factor beta 1 (TGF-beta 1) and total interferon (IFN) protein 
      levels. In addition, RNA was obtained from pleural cells and examined for 
      TGF-beta 1, tumor necrosis factor alpha (TNF-alpha), IFN-gamma, and interleukin-8 
      (IL-8) expression by real-time PCR. Finally, pleural cells were examined for the 
      ability to proliferate in response to concanavalin A and purified protein 
      derivative (PPD) in vitro. In the pleural fluid, TGF-beta 1 protein 
      concentrations increased over the course of the inflammatory response while IFN 
      protein levels were not significantly altered. Expression of TGF-beta 1 mRNA 
      peaked on days 3 and 4, and IFN-gamma mRNA expression peaked on day 3 and then 
      returned to background levels. TNF-alpha mRNA expression was highest on days 2 to 
      4, and IL-8 mRNA levels remained elevated between days 2 and 5, peaking on day 3 
      before returning to background levels. PPD-induced proliferative responses were 
      evident by day 3 and remained present throughout the study. Analysis of cytokine 
      expression during tuberculous pleurisy may lead to a better understanding of the 
      self-healing nature of this manifestation of tuberculosis.
FAU - Allen, Shannon Sedberry
AU  - Allen SS
AD  - Department of Medical Microbiology & Immunology, Texas A&M University System 
      Health Science Center, College Station, Texas 77843, USA. 
      sedberry@medicine.tamu.edu
FAU - McMurray, David N
AU  - McMurray DN
LA  - eng
GR  - R01 AI015495/AI/NIAID NIH HHS/United States
GR  - R01 AI 15495/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tuberculin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11028-71-0 (Concanavalin A)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Concanavalin A/pharmacology
MH  - Cytokines/*genetics/metabolism
MH  - Gene Expression
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Interferon-gamma/genetics
MH  - Interferons/metabolism
MH  - Interleukin-8/genetics
MH  - Lymphocyte Activation/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Time Factors
MH  - Transforming Growth Factor beta/genetics/metabolism
MH  - Transforming Growth Factor beta1
MH  - Tuberculin/pharmacology
MH  - Tuberculosis, Pleural/etiology/*genetics/*immunology
MH  - Tumor Necrosis Factor-alpha/genetics
PMC - PMC166002
EDAT- 2003/07/23 05:00
MHDA- 2003/08/27 05:00
PMCR- 2003/08/01
CRDT- 2003/07/23 05:00
PHST- 2003/07/23 05:00 [pubmed]
PHST- 2003/08/27 05:00 [medline]
PHST- 2003/07/23 05:00 [entrez]
PHST- 2003/08/01 00:00 [pmc-release]
AID - 0226 [pii]
AID - 10.1128/IAI.71.8.4271-4277.2003 [doi]
PST - ppublish
SO  - Infect Immun. 2003 Aug;71(8):4271-7. doi: 10.1128/IAI.71.8.4271-4277.2003.