PMID- 12874783
OWN - NLM
STAT- MEDLINE
DCOM- 20031031
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 38
IP  - 1
DP  - 2003 Sep
TI  - Aneuploidy in oncocytic lesions of the thyroid gland: diffuse accumulation of 
      mitochondria within the cell is associated with trisomy 7 and progressive 
      numerical chromosomal alterations.
PG  - 22-31
AB  - Oncocytic cells are characterized by a greatly increased number of mitochondria 
      that distend the cell cytoplasm and result in a distinctive granular appearance 
      of the cell on conventional histology sections. Oncocytes are frequently found in 
      metabolically active human tissues including the thyroid gland, and, as a general 
      rule, when their proportion in a thyroid tumor is greater than 75% the tumor is 
      referred to as oncocytic (Hurthle cell) adenoma or carcinoma. Such tumors 
      represent a subset of thyroid lesions, and recently, both interphase fluorescence 
      in situ hybridization (FISH) and comparative genomic hybridization (CGH) studies 
      reported that they may show aneuploidy, with widespread numerical chromosomal 
      alterations. In contrast, very few cases have been studied by conventional 
      cytogenetic analysis. Whether the cells with chromosomal changes are the same as 
      those with mitochondrial accumulation or whether lesions only partially composed 
      of oncocytic cells also have cytogenetic alterations is unclear. To investigate 
      the relationship between acquisition of the oncocytic phenotype and numerical 
      chromosomal changes, we analyzed a random selection of thyroid lesions with (18 
      cases) and without (11 cases) morphological evidence of oncocytic 
      differentiation. Lesions with oncocytes included hyperplastic nodules, adenomas, 
      Hurthle cell tumors, and papillary carcinomas with lymphocytic stroma 
      (Whartin-like tumors of the thyroid). Karyotypic changes were analyzed by 
      cytogenetic analysis, FISH, or CGH, and the results were compared with in situ 
      analysis of mitochondrial accumulation by immunofluorescence. A striking 
      correlation between the presence of oncocytes and the presence of aneuploid 
      katyotypes was seen in the oncocytic follicular thyroid nodules, but not in the 
      oncocytic papillary tumors. Structural chromosome changes or normal karyotypes 
      were observed in the lesions lacking oncocytic features. Extending the FICTION 
      technique to the evaluation of a cytoplasmic antigen (mitochondrial membrane 
      antigen), we pursued the simultaneous visualization of both mitochondrial 
      increase and numerical chromosomal alterations, and showed that oncocytes of 
      follicular lesions are prone to become aneuploid. Our data support the contention 
      that follicular tumors composed of oncocytes should be regarded as a distinct 
      subset.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Dettori, Tinuccia
AU  - Dettori T
AD  - Department of Biomedical Sciences and Technology, University of Cagliari, 
      Cagliari, Italy.
FAU - Frau, Daniela V
AU  - Frau DV
FAU - Lai, Maria L
AU  - Lai ML
FAU - Mariotti, Stefano
AU  - Mariotti S
FAU - Uccheddu, Alessandro
AU  - Uccheddu A
FAU - Daniele, Giovanni M
AU  - Daniele GM
FAU - Tallini, Giovanni
AU  - Tallini G
FAU - Faa, Gavino
AU  - Faa G
FAU - Vanni, Roberta
AU  - Vanni R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Chromosome Disorders/*genetics
MH  - Chromosomes, Human, Pair 7/*genetics
MH  - Cytogenetic Analysis/methods
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Middle Aged
MH  - Mitochondria/*genetics/metabolism/pathology
MH  - Nucleic Acid Hybridization/methods
MH  - Oxyphil Cells/metabolism/*pathology
MH  - Thyroid Neoplasms/*genetics/metabolism
MH  - Trisomy/*genetics
EDAT- 2003/07/23 05:00
MHDA- 2003/11/01 05:00
CRDT- 2003/07/23 05:00
PHST- 2003/07/23 05:00 [pubmed]
PHST- 2003/11/01 05:00 [medline]
PHST- 2003/07/23 05:00 [entrez]
AID - 10.1002/gcc.10238 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2003 Sep;38(1):22-31. doi: 10.1002/gcc.10238.