PMID- 12874943
OWN - NLM
STAT- MEDLINE
DCOM- 20030806
LR  - 20070724
IS  - 0903-465X (Print)
IS  - 0903-465X (Linking)
IP  - 109
DP  - 2003
TI  - The intramural innervation of the rabbit upper urinary tract.
PG  - 18-22
AB  - The role of the autonomic innervation of the upper urinary tract for 
      pyeloureteral motility is not completely understood. It is still debatable if the 
      autonomic nervous system might play a modulating role on the ureteral 
      peristalsis. The aim of this study was to investigate the distribution and 
      regional variation of the intramural innervation using whole-mount preparations 
      of the rabbit upper urinary tract. Whole-mount preparation was performed at upper 
      urinary tracts of healthy rabbits. Immunohistochemistry was employed using 
      Neurofilament (NF), Tyrosine Hydroxylase (TH), Choline Acetyltransferase (ChAT) 
      and Substance P (SP) antibodies. NADPH-diaphorase and Acetylcholinesterase (AChE) 
      histochemistry was carried out at the specimens. The stains were evaluated using 
      brightfield, fluorescence and laser confocal microscopy. NF-, TH-, ChAT- and 
      SP-immunoreactive (-IR) nerves formed distinct neuronal plexuses at the 
      submucosal and muscle layers. Perivascular TH-, ChAT- and SP-IR fibres were 
      demonstrated. AChE positive nerves were revealed in all layers, but only moderate 
      NADPH-diaphorase positive innervation was found. Renal pelvis, upper and lower 
      ureter showed enriched intrinsic innervation. Ganglia were found at the 
      ureteropelvic border and the distal ureter. Whole-mount preparation technique 
      revealed detailed informations about morphology and regional variation of the 
      intramural innervation of the rabbit upper urinary tract.
FAU - Rolle, Udo
AU  - Rolle U
AD  - Department of Paediatric Surgery, University of Leipzig, Oststr. 21-25, 04317 
      Leipzig, Germany. udorolle@yahoo.com
FAU - Brylla, Elke
AU  - Brylla E
FAU - Chertin, Boris
AU  - Chertin B
FAU - Cascio, Salvatore
AU  - Cascio S
FAU - Puri, Prem
AU  - Puri P
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - APMIS Suppl
JT  - APMIS. Supplementum
JID - 8812090
RN  - 33507-63-0 (Substance P)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 1.6.99.1 (NADPH Dehydrogenase)
RN  - EC 2.3.1.6 (Choline O-Acetyltransferase)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - Choline O-Acetyltransferase/*metabolism
MH  - Immunohistochemistry
MH  - NADPH Dehydrogenase/metabolism
MH  - Rabbits
MH  - Substance P/metabolism
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Urinary Tract/*innervation/metabolism
EDAT- 2003/07/24 05:00
MHDA- 2003/08/07 05:00
CRDT- 2003/07/24 05:00
PHST- 2003/07/24 05:00 [pubmed]
PHST- 2003/08/07 05:00 [medline]
PHST- 2003/07/24 05:00 [entrez]
PST - ppublish
SO  - APMIS Suppl. 2003;(109):18-22.