PMID- 12875291
OWN - NLM
STAT- MEDLINE
DCOM- 20030731
LR  - 20081121
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 68
IP  - 4
DP  - 2003 Apr
TI  - Ex vivo interferon-gamma immune response to thrombospondin-related adhesive 
      protein in coastal Kenyans: longevity and risk of Plasmodium falciparum 
      infection.
PG  - 421-30
AB  - Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum is 
      currently being tested in human vaccine studies. However, its natural reactivity 
      in the field remains poorly characterized. More than 40% of 217 Kenyan donors 
      responded in an ex vivo interferon-gamma (IFN-gamma) enzyme-linked immunospot 
      (ELISPOT) assay to at least one of 14 20mer peptides spanning 42% of the antigen. 
      Reactivity was comparable from early childhood (>1 year of age) to old age, and 
      the maximal precursor frequency of TRAP-specific cells to all 14 peptides was 1 
      in 4,000. Prospective follow-up for one year indicated that these low-level ex 
      vivo responses to TRAP did not protect against the subsequent development of 
      malaria. Retesting of selected donors after one year showed a complete change in 
      the reactivity pattern, suggesting that malaria-specific ex vivo IFN-gamma 
      ELISPOT assay responses are short lived in naturally exposed donors, even to 
      conserved epitopes. This study provides important information regarding natural 
      reactivity to a key malaria antigen.
FAU - Flanagan, Katie L
AU  - Flanagan KL
AD  - Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe 
      Hospital, Headington, Oxford, United Kingdom. katie.flanagan@lshtm.ac.uk
FAU - Mwangi, Tabitha
AU  - Mwangi T
FAU - Plebanski, Magdalena
AU  - Plebanski M
FAU - Odhiambo, Kennedy
AU  - Odhiambo K
FAU - Ross, Amanda
AU  - Ross A
FAU - Sheu, Eric
AU  - Sheu E
FAU - Kortok, Moses
AU  - Kortok M
FAU - Lowe, Brett
AU  - Lowe B
FAU - Marsh, Kevin
AU  - Marsh K
FAU - Hill, Adrian V S
AU  - Hill AV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (Malaria Vaccines)
RN  - 0 (Peptide Fragments)
RN  - 0 (Protozoan Proteins)
RN  - 120300-02-9 (thrombospondin-related adhesive protein, protozoan)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Amino Acid Sequence
MH  - Child
MH  - Child, Preschool
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Immunity, Cellular
MH  - Infant
MH  - Interferon-gamma/*biosynthesis/chemistry
MH  - Kenya/epidemiology
MH  - Leukocytes, Mononuclear/immunology
MH  - Longevity
MH  - Malaria Vaccines/*standards
MH  - Malaria, Falciparum/epidemiology/immunology/*prevention & control
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Peptide Fragments/immunology
MH  - Pilot Projects
MH  - Protozoan Proteins/chemistry/*immunology
MH  - Risk Factors
MH  - T-Lymphocytes/immunology
EDAT- 2003/07/24 05:00
MHDA- 2003/08/02 05:00
CRDT- 2003/07/24 05:00
PHST- 2003/07/24 05:00 [pubmed]
PHST- 2003/08/02 05:00 [medline]
PHST- 2003/07/24 05:00 [entrez]
PST - ppublish
SO  - Am J Trop Med Hyg. 2003 Apr;68(4):421-30.