PMID- 12875522
OWN - NLM
STAT- MEDLINE
DCOM- 20030910
LR  - 20081121
IS  - 0036-5548 (Print)
IS  - 0036-5548 (Linking)
VI  - 35
IP  - 5
DP  - 2003
TI  - Differential HLA gene expression in measles vaccine seropositive and seronegative 
      subjects: a pilot study.
PG  - 332-6
AB  - This is the first study using GeneChip technology to elucidate genetic 
      determinants of the measles vaccine response. A comparative gene expression study 
      was conducted using Affymetrix's Human GeneChip U-95A in 5 human subjects 
      immunized with a 'booster' dose of measles vaccine (Attenuax, Merck) to determine 
      whether serologically distinct subjects exhibit differential expression of human 
      leukocyte antigen (HLA) genes. Healthy individuals aged 15-25 y, previously 
      immunized with 2 doses of measles-mumps-rubella-II (MMR-II) vaccine, were 
      classified as measles vaccine immunoglobulin G-specific antibody seronegatives (n 
      = 2) and seropositives (n = 3). Changes in expression of HLA genes in 
      seronegatives and seropositives were studied on days 7 and 14 post-measles 
      vaccination using Microarray Suite 5.0 (MAS 5.0). There was increased expression 
      of the HLA class I-B (p = 0.0002), HLA class II cluster of DMA, DMB, TAP1, TAP2 
      (p = 0.0007) and HLA-DR (p = 0.0001) genes, and decreased expression of HLA class 
      I MICB molecule (p = 1), HLA class I-A (p = 0.9999) and major histocompatibility 
      complex class III HSP 70 (p = 0.9999) genes on day 7 or day 14 postvaccination in 
      seropositives compared with seronegatives. These results suggest an association 
      between antibody response and differential HLA gene activation and may explain 
      one potential mechanism underlying measles vaccine non-response.
FAU - Dhiman, Neelam
AU  - Dhiman N
AD  - Mayo Vaccine Research Group, Mayo Clinic, Rochester, Minnesota 55905, USA.
FAU - Bonilla, Ruben G
AU  - Bonilla RG
FAU - Jacobson, Robert M
AU  - Jacobson RM
FAU - O'Kane, Dennis
AU  - O'Kane D
FAU - Poland, Gregory A
AU  - Poland GA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Scand J Infect Dis
JT  - Scandinavian journal of infectious diseases
JID - 0215333
RN  - 0 (HLA Antigens)
RN  - 0 (Measles Vaccine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Expression Regulation, Viral
MH  - Genes, MHC Class I/*genetics
MH  - Genes, MHC Class II/*genetics
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - Immunity/physiology
MH  - Male
MH  - Measles/*immunology/*prevention & control
MH  - Measles Vaccine/*administration & dosage
MH  - Pilot Projects
MH  - Sensitivity and Specificity
MH  - Seroepidemiologic Studies
MH  - Transcriptional Activation
MH  - Vaccination/methods
EDAT- 2003/07/24 05:00
MHDA- 2003/09/11 05:00
CRDT- 2003/07/24 05:00
PHST- 2003/07/24 05:00 [pubmed]
PHST- 2003/09/11 05:00 [medline]
PHST- 2003/07/24 05:00 [entrez]
AID - BFPKNFG9DBMVH47B [pii]
AID - 10.1080/00365540310007105 [doi]
PST - ppublish
SO  - Scand J Infect Dis. 2003;35(5):332-6. doi: 10.1080/00365540310007105.