PMID- 12876367
OWN - NLM
STAT- MEDLINE
DCOM- 20040420
LR  - 20190605
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 59
IP  - Pt 8
DP  - 2003 Aug
TI  - Cloning, expression, purification, crystallization and preliminary X-ray 
      diffraction analysis of the structural domain of the nucleocapsid N protein from 
      porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 1504-6
AB  - The structural domain of the PRRSV nucleocapsid N protein was overexpressed in 
      Escherichia coli and purified to homogeneity. Crystals of the expressed protein, 
      designated His-Ndelta(57), were obtained by hanging-drop vapour diffusion using 
      PEG 3350 as precipitant at pH 6.5. A native data set from a frozen crystal was 
      collected to 2.7 A resolution using synchrotron radiation. The crystals belong to 
      space group P3(1)21 or P3(2)21, with unit-cell parameters a = 44.41, c = 125.05, 
      and contain a dimer in the asymmetric unit.
FAU - Doan, Danny N P
AU  - Doan DN
AD  - Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, 
      Singapore. doan@imcb.nus.edu.sg
FAU - Dokland, Terje
AU  - Dokland T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030723
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - 0 (DNA, Complementary)
RN  - 0 (Nucleocapsid Proteins)
RN  - 4QD397987E (Histidine)
SB  - IM
MH  - Cloning, Molecular
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - DNA, Complementary/metabolism
MH  - Dimerization
MH  - Escherichia coli/metabolism
MH  - Histidine/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Nucleocapsid Proteins/*chemistry
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism
MH  - Protein Structure, Tertiary
MH  - X-Ray Diffraction
EDAT- 2003/07/24 05:00
MHDA- 2004/04/21 05:00
CRDT- 2003/07/24 05:00
PHST- 2003/03/31 00:00 [received]
PHST- 2003/06/09 00:00 [accepted]
PHST- 2003/07/24 05:00 [pubmed]
PHST- 2004/04/21 05:00 [medline]
PHST- 2003/07/24 05:00 [entrez]
AID - S0907444903012721 [pii]
AID - 10.1107/s0907444903012721 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1504-6. doi: 
      10.1107/s0907444903012721. Epub 2003 Jul 23.