PMID- 12876458
OWN - NLM
STAT- MEDLINE
DCOM- 20031107
LR  - 20191026
IS  - 0920-8569 (Print)
IS  - 0920-8569 (Linking)
VI  - 26
IP  - 3
DP  - 2003 May
TI  - Complete genomic sequence of the Australian south-west genotype of Sindbis virus: 
      comparisons with other Sindbis strains and identification of a unique deletion in 
      the 3'-untranslated region.
PG  - 317-27
AB  - Our previous studies have shown that two distinct genotypes of Sindbis (SIN) 
      virus occur in Australia. One of these, the Oriental/Australian type, circulates 
      throughout most of the Australian continent, whereas the recently identified 
      south-west (SW) genetic type appears to be restricted to a distinct geographic 
      region located in the temperate south-west of Australia. We have now determined 
      the complete nucleotide and translated amino acid sequences of a SW isolate of 
      SIN virus (SW6562) and performed comparative analyses with other SIN viruses at 
      the genomic level. The genome of SW6562 is 11,569 nucleotides in length, 
      excluding the cap nucleotide and poly (A) tail. Overall this virus differs from 
      the prototype SIN virus (strain AR339) by 23% in nucleotide sequence and 12.5% in 
      amino acid sequence. Partial sequences of four regions of the genome of four SW 
      isolates were determined and compared with the corresponding sequences from a 
      number of SIN isolates from different regions of the World. These regions are the 
      non-structural protein (nsP3), the E2 gene, the capsid gene, and the repeated 
      sequence elements (RSE) of the 3'UTR. These comparisons revealed that the SW SIN 
      viruses were more closely related to South African and European strains than to 
      other Australian isolates of SIN virus. Thus the SW genotype of SIN virus may 
      have been introduced into this region of Australia by viremic humans or migratory 
      birds and subsequently evolved independently in the region. The sequence data 
      also revealed that the SW genotype contains a unique deletion in the RSE of the 
      3'UTR region of the genome. Previous studies have shown that deletions in this 
      region of the SIN genome can have significant effects on virus replication in 
      mosquito and avian cells, which may explain the restricted distribution of this 
      genotype of SIN virus.
FAU - Saleh, Suha M
AU  - Saleh SM
AD  - Department of Microbiology, School of Molecular and Microbial Sciences, 
      University of Queensland, Brisbane, Australia.
FAU - Poidinger, Michael
AU  - Poidinger M
FAU - Mackenzie, John S
AU  - Mackenzie JS
FAU - Broom, Annette K
AU  - Broom AK
FAU - Lindsay, Michael D
AU  - Lindsay MD
FAU - Hall, Roy A
AU  - Hall RA
LA  - eng
SI  - GENBANK/AF061237
SI  - GENBANK/AF061238
SI  - GENBANK/AF061239
SI  - GENBANK/AF429428
SI  - GENBANK/AF439978
SI  - GENBANK/AF439979
SI  - GENBANK/AF439980
SI  - GENBANK/AF439981
SI  - GENBANK/AF439982
SI  - GENBANK/AF439983
SI  - GENBANK/AF439984
SI  - GENBANK/AF439985
SI  - GENBANK/AF439986
SI  - GENBANK/AF439987
SI  - GENBANK/AF439988
SI  - GENBANK/AF439989
SI  - GENBANK/AF439990
SI  - GENBANK/AF439991
SI  - GENBANK/AF450099
SI  - GENBANK/AF450104
SI  - GENBANK/AF450105
SI  - GENBANK/AF450106
SI  - GENBANK/AF450107
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Genome, Viral
MH  - Genotype
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - *Sequence Analysis, DNA
MH  - Sindbis Virus/*classification/*genetics
MH  - Western Australia
EDAT- 2003/07/24 05:00
MHDA- 2003/11/08 05:00
CRDT- 2003/07/24 05:00
PHST- 2003/07/24 05:00 [pubmed]
PHST- 2003/11/08 05:00 [medline]
PHST- 2003/07/24 05:00 [entrez]
AID - 5127078 [pii]
AID - 10.1023/a:1024407516352 [doi]
PST - ppublish
SO  - Virus Genes. 2003 May;26(3):317-27. doi: 10.1023/a:1024407516352.