PMID- 12878353
OWN - NLM
STAT- MEDLINE
DCOM- 20040312
LR  - 20190906
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 64
IP  - 8
DP  - 2003 Aug
TI  - Soluble HLA-G inhibits human dendritic cell-triggered allogeneic T-cell 
      proliferation without altering dendritic differentiation and maturation 
      processes.
PG  - 752-61
AB  - The role of the nonclassical human leukocyte antigen (HLA) class Ib molecule 
      HLA-G in immune tolerance was first reported at maternofetal interface. This 
      immunomodulating role could be exerted more generally in tumoral or 
      post-transplantation situations in inhibiting natural killer (NK) and 
      T-lymphocyte mediated lysis. Among the different transcripts resulting from 
      alternative splicing, the mainly secreted isoform, HLA-G5, corresponds to 
      complete molecule and has been demonstrated to be elevated in melanomas and in 
      serum from heart-transplanted patients. As dendritic cells expressed ILT4, an 
      inhibitory receptor capable of interacting with HLA-G, we have studied the effect 
      of soluble HLA-G (HLA-G5) on differentiation, maturation, apoptosis and function 
      of monocyte or CD34+-derived dendritic cells (DC). Soluble HLA-G did not alter 
      differentiation, maturation or apoptosis of DC whatever their origin. On the 
      other hand, an inhibitory effect of HLA-G5 on T lymphocytes proliferation was 
      found in 53% of mixed leukocyte reactions (MLR) and was variable in intensity. 
      These data demonstrate an indirect way of HLA-G5 action on DC occurring via T 
      lymphocytes that reinforces the immune inhibitory role of soluble HLA-G capable 
      to be secreted during tumoral malignancies or following heart transplantation.
FAU - Le Friec, Gaelle
AU  - Le Friec G
AD  - Laboratoire Universitaire d'Hematologie et de la Biologie des Cellules Sanguines, 
      UPRES EA 22-33, Faculte de Medecine, Universite de Rennes 1, Rennes, France.
FAU - Laupeze, Beatrice
AU  - Laupeze B
FAU - Fardel, Olivier
AU  - Fardel O
FAU - Sebti, Yasmine
AU  - Sebti Y
FAU - Pangault, Celine
AU  - Pangault C
FAU - Guilloux, Valerie
AU  - Guilloux V
FAU - Beauplet, Alain
AU  - Beauplet A
FAU - Fauchet, Renee
AU  - Fauchet R
FAU - Amiot, Laurence
AU  - Amiot L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, CD34)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Antigens, CD34/analysis
MH  - *Apoptosis
MH  - Blotting, Western
MH  - *Cell Differentiation
MH  - Cell Line, Tumor
MH  - Dendritic Cells/immunology/metabolism/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HLA Antigens/genetics/*physiology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/genetics/*physiology
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/physiology
MH  - Phenotype
MH  - Solubility
MH  - T-Lymphocytes/*physiology
EDAT- 2003/07/25 05:00
MHDA- 2004/03/16 05:00
CRDT- 2003/07/25 05:00
PHST- 2003/07/25 05:00 [pubmed]
PHST- 2004/03/16 05:00 [medline]
PHST- 2003/07/25 05:00 [entrez]
AID - S0198885903000910 [pii]
AID - 10.1016/s0198-8859(03)00091-0 [doi]
PST - ppublish
SO  - Hum Immunol. 2003 Aug;64(8):752-61. doi: 10.1016/s0198-8859(03)00091-0.