PMID- 12881298
OWN - NLM
STAT- MEDLINE
DCOM- 20040217
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 103
IP  - 1
DP  - 2004 Jan 1
TI  - Little evidence of donor-derived epithelial cells in early digestive acute 
      graft-versus-host disease.
PG  - 360-2
AB  - Donor origin of epithelial intestinal cells has been studied in animals and 
      humans after transplantation and has been used as evidence of hematopoietic stem 
      cell (HSC) plasticity. However, in the human gastrointestinal tract, no study 
      used X- or Y-chromosome detection by fluorescence in situ hybridization (FISH) 
      coupled with immunologic stainings to characterize cell types on the same tissue 
      section. Here, we combined these techniques on the same section of duodenal 
      epithelium in 6 patients with acute graft-versus-host disease. Donor-derived 
      lymphoid cells were detected in the epithelium and the lamina propria, as 
      expected. However, using our stringent criteria, no donor-derived cells could be 
      proven to be epithelial.
FAU - Meignin, Veronique
AU  - Meignin V
AD  - Institut National de la Sante et de la Recherche Medicale (INSERM), Universite 
      Paris VII, Hopital Saint Louis, Paris, France.
FAU - Soulier, Jean
AU  - Soulier J
FAU - Brau, Frederic
AU  - Brau F
FAU - Lemann, Marc
AU  - Lemann M
FAU - Gluckman, Eliane
AU  - Gluckman E
FAU - Janin, Anne
AU  - Janin A
FAU - Socie, Gerard
AU  - Socie G
LA  - eng
PT  - Journal Article
DEP - 20030724
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Case-Control Studies
MH  - Chromosomes, Human, X/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - Duodenum/*immunology/*pathology
MH  - Epithelial Cells/immunology/pathology
MH  - Female
MH  - Graft vs Host Disease/genetics/*immunology/*pathology
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/immunology/therapy
MH  - Male
MH  - Tissue Donors
MH  - Transplantation, Homologous
EDAT- 2003/07/26 05:00
MHDA- 2004/02/18 05:00
CRDT- 2003/07/26 05:00
PHST- 2003/07/26 05:00 [pubmed]
PHST- 2004/02/18 05:00 [medline]
PHST- 2003/07/26 05:00 [entrez]
AID - S0006-4971(20)50327-3 [pii]
AID - 10.1182/blood-2003-06-1843 [doi]
PST - ppublish
SO  - Blood. 2004 Jan 1;103(1):360-2. doi: 10.1182/blood-2003-06-1843. Epub 2003 Jul 
      24.