PMID- 12882781
OWN - NLM
STAT- MEDLINE
DCOM- 20030811
LR  - 20220408
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 44
IP  - 8
DP  - 2003 Aug
TI  - Presence and localization of neurotrophins and neurotrophin receptors in rat 
      lacrimal gland.
PG  - 3352-7
AB  - PURPOSE: To determine which of the neurotrophins (NTs)-nerve growth factor (NGF), 
      brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3), and 
      neurotrophin-4/5 (NT4)-and their receptors (NTrs), TrkA, TrkB, TrkC, and p75, are 
      present in the adult rat lacrimal gland. METHODS: RT-PCR was performed on RNA 
      isolated from male rat lacrimal gland, using oligonucleotides specific to each NT 
      and NTr. The presence of NT and NTr protein, was determined by Western blot 
      analysis of lacrimal gland homogenate or membranes. The location of NTs and NTrs 
      was determined by immunofluorescence histochemistry. Western blot analyses and 
      immunofluorescence microscopy were performed using primary rabbit polyclonal 
      antibodies raised against NTs and NTrs. RESULTS: RT-PCR showed positive bands at 
      the appropriate sizes for NGF, BDNF, NT3, and NT4, and for the receptors TrkA, 
      TrkB, TrkC, and p75. Western blot analysis confirmed these results, showing that 
      the lacrimal gland expresses NGF, BDNF, NT3, and NT4 as well as the NTrs TrkA, 
      TrkB, and TrkC and the p75 protein. NGF, BDNF, NT3, and NT4 were localized in the 
      lacrimal gland acini with differing cellular distributions, whereas TrkA, TrkB, 
      and TrkC, were localized in myoepithelial cell and ductal cell membranes. The 
      protein p75 was expressed only on myoepithelial cell membranes. CONCLUSIONS: 
      Members of the neurotrophin family of growth factors and their receptors are 
      present in rat lacrimal gland, which suggests a role for NTs and their receptors 
      in the lacrimal gland.
FAU - Ghinelli, Emiliano
AU  - Ghinelli E
AD  - Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical 
      School, Boston, Massachusetts. USA. eghinelli@vision.eri.harvard.edu
FAU - Johansson, Jenny
AU  - Johansson J
FAU - Rios, Jose D
AU  - Rios JD
FAU - Chen, Li-Li
AU  - Chen LL
FAU - Zoukhri, Driss
AU  - Zoukhri D
FAU - Hodges, Robin R
AU  - Hodges RR
FAU - Dartt, Darlene A
AU  - Dartt DA
LA  - eng
GR  - R01 EY006177/EY/NEI NIH HHS/United States
GR  - EY06177/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (DNA Primers)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - DNA Primers/chemistry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Lacrimal Apparatus/*metabolism
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Nerve Growth Factors/genetics/*metabolism
MH  - RNA/isolation & purification
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Nerve Growth Factor/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/07/29 05:00
MHDA- 2003/08/12 05:00
CRDT- 2003/07/29 05:00
PHST- 2003/07/29 05:00 [pubmed]
PHST- 2003/08/12 05:00 [medline]
PHST- 2003/07/29 05:00 [entrez]
AID - 10.1167/iovs.03-0037 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3352-7. doi: 10.1167/iovs.03-0037.