PMID- 12882803
OWN - NLM
STAT- MEDLINE
DCOM- 20030811
LR  - 20220408
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 44
IP  - 8
DP  - 2003 Aug
TI  - Transplantation of syngeneic Schwann cells to the retina of the rhodopsin 
      knockout (rho(-/-)) mouse.
PG  - 3526-32
AB  - PURPOSE: To determine whether subretinal Schwann cell transplantation can prolong 
      the survival of photoreceptors in the rhodopsin knockout (rho(-/-)) mouse. 
      METHODS: Schwann cells were prepared from postnatal day (PN) 5 to 7 mouse pups 
      and grafted subretinally into the eyes of PN35 rho(-/-) mice. RT-PCR was 
      performed on similarly prepared cells to determine growth factor production in 
      vitro. Eyes were retrieved at PN70 for anatomic and statistical analysis. Control 
      animals received grafts of fibroblasts or sham surgery. RESULTS: RT-PCR 
      demonstrated the presence of message for ciliary neurotrophic factor (CNTF), 
      brain-derived neurotrophic factor (BDNF), and glia-derived neurotrophic factor 
      (GDNF) in the cultured Schwann cells. Schwann cell grafts produced a 
      statistically significant rescue of photoreceptors in a restricted area of retina 
      at PN70, but the effect was lost by PN140. Preserved inner segments could be 
      identified, but outer segments were never present. Sham surgery also resulted in 
      photoreceptor rescue but at a reduced level. Fibroblast grafts appeared to 
      produce little or no rescue effect. Grafts of Schwann cells or fibroblasts and 
      sham surgery induced a reactive Muller glial response. CONCLUSIONS: Schwann cells 
      can prolong photoreceptor survival in the rhodopsin knockout mouse until at least 
      PN70.
FAU - Keegan, David J
AU  - Keegan DJ
AD  - Department of Pathology, Institute of Ophthalmology, London, United Kingdom. 
      d.keegan@ucl.ac.uk
FAU - Kenna, Paul
AU  - Kenna P
FAU - Humphries, Marian M
AU  - Humphries MM
FAU - Humphries, Peter
AU  - Humphries P
FAU - Flitcroft, Daniel I
AU  - Flitcroft DI
FAU - Coffey, Peter J
AU  - Coffey PJ
FAU - Lund, Ray D
AU  - Lund RD
FAU - Lawrence, Jean M
AU  - Lawrence JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Gdnf protein, mouse)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 9009-81-8 (Rhodopsin)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Count
MH  - Cell Survival
MH  - Cell Transplantation
MH  - Ciliary Neurotrophic Factor/genetics/metabolism
MH  - Fibroblasts/transplantation
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Growth Factors/genetics/metabolism
MH  - Photoreceptor Cells, Vertebrate/pathology
MH  - RNA, Messenger/metabolism
MH  - Retina/pathology/*surgery
MH  - Retinal Degeneration/genetics/pathology/*surgery
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rhodopsin/*genetics
MH  - Schwann Cells/metabolism/*transplantation
MH  - Sciatic Nerve/cytology
MH  - Transplantation, Isogeneic
EDAT- 2003/07/29 05:00
MHDA- 2003/08/12 05:00
CRDT- 2003/07/29 05:00
PHST- 2003/07/29 05:00 [pubmed]
PHST- 2003/08/12 05:00 [medline]
PHST- 2003/07/29 05:00 [entrez]
AID - 10.1167/iovs.02-0097 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2003 Aug;44(8):3526-32. doi: 10.1167/iovs.02-0097.