PMID- 12883706
OWN - NLM
STAT- MEDLINE
DCOM- 20040421
LR  - 20131121
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 10
IP  - 5
DP  - 2003 Sep-Oct
TI  - Rho regulates the hepatocyte growth factor/scatter factor-stimulated cell 
      motility of human oral squamous cell carcinoma cells.
PG  - 1351-6
AB  - To investigate the effects of hepatocyte growth factor/scatter factor (HGF/SF) on 
      the invasion and metastasis of human oral squamous cell carcinoma (SCC) cells, we 
      examined cell motility and intercellular signal transduction of a human oral SCC 
      cell line (SAS) obtained from the primary lesion of a tongue carcinoma. HGF/SF 
      stimulation significantly enhanced the motility of SAS cells in a dose-dependent 
      manner. Clostridium botulinum C3 exoenzyme (C3), which is known to selectively 
      impair the function of Ras-related small G-protein p21rho (Rho), significantly 
      reduced the motility of SAS cells. HGF/SF stimulation also enhanced the tyrosine 
      phosphorylation of HGF receptors (c-Met) and focal adhesion kinase (FAK) on SAS 
      cells, but C3 completely inhibited the phosphorylation of FAK. Furthermore, it 
      was observed that Rho A protein, normally located around the nuclear area, was 
      translocated to the membrane and levels in the cytolysate increased following 
      HGF/SF stimulation with no change in Rho A mRNA. These results suggest that the 
      activation of FAK caused by phosphorylation of c-Met may mediate the 
      HGF/SF-induced motility of human oral SCC cells, and that Rho protein regulates 
      the tyrosine phosphorylation of FAK through translocation from the nucleus to the 
      membrane.
FAU - Kitajo, Hiroyuki
AU  - Kitajo H
AD  - Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health 
      Sciences University of Hokkaido, Tobetsu, Ishikari, Hokkaido 061-0293, Japan.
FAU - Shibata, Toshiyuki
AU  - Shibata T
FAU - Nagayasu, Hiroki
AU  - Nagayasu H
FAU - Kawano, Takashi
AU  - Kawano T
FAU - Hamada, Jun-Ichi
AU  - Hamada J
FAU - Yamashita, Tomomi
AU  - Yamashita T
FAU - Arisue, Makoto
AU  - Arisue M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 42HK56048U (Tyrosine)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.4.2.- (ADP Ribose Transferases)
RN  - EC 2.4.2.- (exoenzyme C3, Clostridium botulinum)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (PTK2 protein, human)
RN  - EC 3.4.24.69 (Botulinum Toxins)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - ADP Ribose Transferases/pharmacology
MH  - Blotting, Western
MH  - Botulinum Toxins/pharmacology
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Membrane/metabolism
MH  - Cell Movement
MH  - Cell Nucleus/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Focal Adhesion Kinase 1
MH  - Focal Adhesion Protein-Tyrosine Kinases
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Microscopy, Confocal
MH  - Mouth Neoplasms/*metabolism
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Transport
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Tyrosine/metabolism
MH  - rho GTP-Binding Proteins/metabolism/*physiology
MH  - rhoA GTP-Binding Protein/metabolism
EDAT- 2003/07/29 05:00
MHDA- 2004/04/22 05:00
CRDT- 2003/07/29 05:00
PHST- 2003/07/29 05:00 [pubmed]
PHST- 2004/04/22 05:00 [medline]
PHST- 2003/07/29 05:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2003 Sep-Oct;10(5):1351-6.