PMID- 12883724
OWN - NLM
STAT- MEDLINE
DCOM- 20040421
LR  - 20051117
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 10
IP  - 5
DP  - 2003 Sep-Oct
TI  - Protein expression of p53 and Bcl-2 has a strong correlation with radiation 
      resistance of laryngeal squamous cell carcinoma but does not predict the 
      radiation failure before treatment.
PG  - 1461-6
AB  - To examine what factors can precisely predict the radio-sensitivity or 
      radio-resistance of early stage laryngeal squamous cell carcinomas (LSCC), 
      protein expression of p53, Bcl-2 and Bax and loss of heterozygosity (LOH) at 3p 
      and 9p loci were investigated. From June 1994 through June 1999, specimens of 
      primary tumors were obtained by biopsy from 21 patients diagnosed as early stage 
      LSCC at Miyagi Cancer Center Hospital under approved protocol. Labeling indexes 
      of p53 and Bcl-2 were markedly increased in the recurrent tumors (n=8) and the 
      differences were significant by statistical analysis (p=0.043 and p=0.015). 
      However, when labeling indexes of the samples before treatment from non-recurrent 
      cases were compared with those of the samples before treatment from recurrent 
      tumors (n=13), no significant difference was observed. When LOH was examined, 16 
      out of these 21 cases were acceptable for evaluation, 4 of which (25%) had a 
      relapse. Allelic loss at the 9p21 and 3p21 loci was found in 6 of 13 cases (46%) 
      and 10 of 16 cases (63%), respectively. The frequency of LOH at the 3p21 locus in 
      the recurrent cases was 100% (4 out of 4) and that in non-recurrent cases was 50% 
      (6 out of 12). The frequency of LOH in recurrent tumors was apparently higher 
      than that in non-recurrent tumors. Preservation rates of the larynx of the 
      patients with 3p21 LOH negative tumors was very high (100%) and that of the 
      patients with 3p21 LOH positive tumors was low. These results indicate that 
      although expression of p53 and Bcl-2 had a strong correlation with recurrent LSCC 
      treated by irradiation, immunohistochemical analysis of p53 and Bcl-2 in the 
      sample before treatment was not able to predict the radiation failure. Moreover, 
      our results also indicated that molecular research on genetic instability such as 
      allelic loss may be more sensitive for the detection of radio-resistant tumor 
      cells.
FAU - Ogawa, Takenori
AU  - Ogawa T
AD  - Department of Otolaryngology, Head and Neck, Tohoku University School of 
      Medicine, Sendai 980-8574, Japan.
FAU - Shiga, Kiyoto
AU  - Shiga K
FAU - Tateda, Masaru
AU  - Tateda M
FAU - Saijo, Shigeru
AU  - Saijo S
FAU - Suzuki, Takashi
AU  - Suzuki T
FAU - Sasano, Hironobu
AU  - Sasano H
FAU - Miyagi, Taeko
AU  - Miyagi T
FAU - Kobayashi, Toshimitsu
AU  - Kobayashi T
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Aged
MH  - Alleles
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*radiotherapy
MH  - *Genes, p53
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/genetics/metabolism/*radiotherapy
MH  - Loss of Heterozygosity
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-bcl-2/*biosynthesis/*genetics
MH  - Recurrence
MH  - Time Factors
MH  - Treatment Outcome
MH  - Tumor Suppressor Protein p53/*biosynthesis
EDAT- 2003/07/29 05:00
MHDA- 2004/04/22 05:00
CRDT- 2003/07/29 05:00
PHST- 2003/07/29 05:00 [pubmed]
PHST- 2004/04/22 05:00 [medline]
PHST- 2003/07/29 05:00 [entrez]
PST - ppublish
SO  - Oncol Rep. 2003 Sep-Oct;10(5):1461-6.