PMID- 12885329
OWN - NLM
STAT- MEDLINE
DCOM- 20040415
LR  - 20141120
IS  - 1090-6576 (Print)
IS  - 1090-6576 (Linking)
VI  - 7
IP  - 2
DP  - 2003 Summer
TI  - Incidence of chromosomal mosaicism in human embryos at different developmental 
      stages analyzed by fluorescence in situ hybridization.
PG  - 85-95
AB  - Chromosomal mosaicism has been reported in in vitro-cultured embryos at early 
      cleavage stages, as well as in morulae and blastocysts. We have assessed the 
      incidence and pattern of mosaicism during in vitro development of human embryos 
      from early-cleavage stages to morula and blastocyst. Fifty spare embryos were 
      fixed for fluorescence in situ hybridization (FISH) analysis for chromosomes X, 
      Y, 13, 18, and 21 on days 2 or 3 (4- to 10-cell stage) (n = 16), on day 4 (morula 
      stage) (n = 14), on day 5 (pre-expanded blastocyst) (n = 5), and the expanded 
      blastocyst stages (n = 15). Blocked embryos (no cleavage observed within the last 
      24 hr) were not included. A total of 2367 cells were analyzed. Four 
      early-cleavage stage embryos were found uniformly diploid; all of the others were 
      mosaic for the chromosomes analyzed (mean diploid nuclei 48.3% +/- 28.7). All of 
      the embryos at more advanced developmental stages, except one fully normal 
      morula, had mosaic chromosome constitutions, with an increase in the percentage 
      of diploid cells in morulae, pre-expanded, and expanded blastocysts, respectively 
      (mean diploid nuclei 78.6% +/- 11.7, 66.0% +/- 20.8, 79.6% +/- 12.8), in 
      comparison with earlier stages. Hypotheses about the origin of mosaicism and 
      embryo regulation mechanisms will be discussed.
FAU - Gonzalez-Merino, Eric
AU  - Gonzalez-Merino E
AD  - Fertility Clinic, Department of Obstetrics and Gynaecology, and Laboratory of 
      Research on Human Reproduction, Erasmus Hospital-ULB, Free University of 
      Brussels, Belgium. ergonzal@ulb.ac.be
FAU - Emiliani, Serena
AU  - Emiliani S
FAU - Vassart, Gilbert
AU  - Vassart G
FAU - Van den Bergh, Marc
AU  - Van den Bergh M
FAU - Vannin, Anne-Sophie
AU  - Vannin AS
FAU - Abramowicz, Marc
AU  - Abramowicz M
FAU - Delneste, Daniele
AU  - Delneste D
FAU - Englert, Yvon
AU  - Englert Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genet Test
JT  - Genetic testing
JID - 9802546
SB  - IM
MH  - Aneuploidy
MH  - Blastocyst/ultrastructure
MH  - Chromosomes, Human/genetics
MH  - Cleavage Stage, Ovum/ultrastructure
MH  - Diploidy
MH  - Embryonic and Fetal Development/*genetics
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - In Vitro Techniques
MH  - Male
MH  - Models, Genetic
MH  - Morula/ultrastructure
MH  - *Mosaicism
EDAT- 2003/07/30 05:00
MHDA- 2004/04/16 05:00
CRDT- 2003/07/30 05:00
PHST- 2003/07/30 05:00 [pubmed]
PHST- 2004/04/16 05:00 [medline]
PHST- 2003/07/30 05:00 [entrez]
AID - 10.1089/109065703322146768 [doi]
PST - ppublish
SO  - Genet Test. 2003 Summer;7(2):85-95. doi: 10.1089/109065703322146768.