PMID- 12885467
OWN - NLM
STAT- MEDLINE
DCOM- 20030821
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 145
IP  - 1
DP  - 2003 Aug
TI  - Insertion of chromosome 11 in chromosome 4 resulting in a 5'MLL-3'AF4 fusion gene 
      in a case of adult acute lymphoblastic leukemia.
PG  - 74-7
AB  - We report on a 69-year-old woman with B-lineage acute lymphoblastic leukemia. 
      Cytogenetic studies at diagnosis with R banding showed an insertion, 
      ins(4;11)(q21;q13q23). Fluorescence in situ hybridization (FISH) with whole 
      chromosome painting probes confirmed the insertion of chromosome 11 material into 
      chromosome 4. FISH using the MLL probe showed the translocation of the 5' end of 
      MLL into chromosome 4. Since the 5'MLL-3'AF4 fusion transcript was detected by a 
      reverse transcriptase polymerase chain reaction, we concluded that the insertion 
      of part of chromosome 11 split the AF4 gene in two, resulting in the presence of 
      the 5'MLL-3'AF4 fusion gene on the der(4) instead of the der(11), as commonly 
      observed. Our findings stress the value of combining banding cytogenetics with 
      FISH and molecular techniques to better assess rearrangements in leukemia.
FAU - Morel, Frederic
AU  - Morel F
AD  - Service de Cytogenetique, Cytologie et Biologie de la Reproduction, CHU Morvan, 
      Brest, France.
FAU - Le Bris, Marie-Josee
AU  - Le Bris MJ
FAU - Douet-Guilbert, Nathalie
AU  - Douet-Guilbert N
FAU - Duchemin, Jerome
AU  - Duchemin J
FAU - Herry, Angele
AU  - Herry A
FAU - Le Calvez, Genevieve
AU  - Le Calvez G
FAU - Marion, Veronique
AU  - Marion V
FAU - Berthou, Christian
AU  - Berthou C
FAU - De Braekeleer, Marc
AU  - De Braekeleer M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (MLL-AF4 fusion protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
SB  - IM
MH  - Aged
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 4
MH  - Female
MH  - Humans
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Oncogene Proteins, Fusion/*analysis
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - *Translocation, Genetic
EDAT- 2003/07/30 05:00
MHDA- 2003/08/22 05:00
CRDT- 2003/07/30 05:00
PHST- 2003/07/30 05:00 [pubmed]
PHST- 2003/08/22 05:00 [medline]
PHST- 2003/07/30 05:00 [entrez]
AID - S0165460803000293 [pii]
AID - 10.1016/s0165-4608(03)00029-3 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2003 Aug;145(1):74-7. doi: 10.1016/s0165-4608(03)00029-3.