PMID- 12889041
OWN - NLM
STAT- MEDLINE
DCOM- 20040316
LR  - 20171116
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 29
IP  - 2
DP  - 2003 Aug
TI  - Detection of a subset of CD30+ anaplastic large cell lymphoma by interphase 
      fluorescence in situ hybridization.
PG  - 61-6
AB  - T/null-cell anaplastic large cell lymphoma (ALCL) is a morphologically and 
      clinically heterogeneous group of non-Hodgkin's lymphoma; to date several 
      morphologic variants have been described on histologic specimens. However, the 
      cytologic features of these variants in the fine-needle aspiration (FNA) 
      specimens have not been well evaluated. The t(2;5)(p23;q35) has been identified 
      in a subset of T/null-ALCL and is known to be associated with a favorable 
      prognosis. We reviewed the cytomorphologic characteristics in 24 FNA specimens of 
      ALCL. In all cases, the diagnosis was confirmed on histologic specimens, and 
      immunohistochemical studies for anaplastic lymphoma kinase (ALK) protein 
      expression were performed on the aspirates. The presence of ALK breakpoints were 
      evaluated in nine cases, using a DNA break-apart probe on chromosome 2 covering 
      the ALK gene by fluorescence in situ hybridization (FISH) techniques. Two hundred 
      cells per case were examined. The results were expressed as the percentage of 
      cells containing more than two signals of chromosome 2 to the total number of 
      cells counted. FNA sites included lymph nodes (20), lung (2), breast (1), and 
      soft tissue (1). The median age of the patients was 56 yr (range, 17-75 yr). 
      Twenty cases had systemic involvement; in four cases, skin was the primary site 
      with secondary involvement of the lymph nodes. All cases were CD30(+) by 
      immunohistochemistry; 20 were of T-cell phenotype and 4 were null cell type. The 
      cytologic evaluation revealed typical anaplastic morphology (common type) with 
      many "hallmark cells" in 16 (67%) cases. Other morphologic variants identified 
      were small cell pattern in five cases, monomorphic pattern in two cases, and 
      lymphohistiocytic pattern in one case. FISH studies showed that six (66.7%) of 
      nine cases had at least two signals of chromosome 2, consistent with ALK 
      breakpoints. With careful cytomorphologic evaluation in conjunction with 
      appropriate immunohistochemical studies, a diagnosis of ALCL can be confidently 
      made in the FNA specimens in the cellular aspirates and its morphologic variants 
      also can be recognized. Furthermore, the FNA specimen is suitable in detecting 
      ALK breakpoints by FISH study, permitting rapid identification of a subset of 
      patients with ALCL, who may have a favorable prognosis. Using a commercially 
      available probe, detection of ALK breakpoints in the FNA specimens is simple and 
      can be a useful diagnostic adjunct in cases where distinction from other 
      lymphomas or lymphoid lesions is morphologically difficult.
CI  - Copyright 2003 Wiley-Liss, Inc.
FAU - Shin, Hyung Ju C
AU  - Shin HJ
AD  - Department of Pathology, University of Texas M.D. Anderson Cancer Center, 
      Houston, Texas, USA. shinhc@msx.upmc.edu
FAU - Thorson, Phataraporn
AU  - Thorson P
FAU - Gu, Jun
AU  - Gu J
FAU - Katz, Ruth L
AU  - Katz RL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Ki-1 Antigen)
RN  - EC 2.7.11.30 (ACVRL1 protein, human)
RN  - EC 2.7.11.30 (Activin Receptors, Type I)
RN  - EC 2.7.11.30 (Activin Receptors, Type II)
SB  - IM
MH  - Activin Receptors, Type I/metabolism
MH  - Activin Receptors, Type II
MH  - Adult
MH  - Aged
MH  - Biopsy, Fine-Needle
MH  - Chromosome Breakage
MH  - *Chromosomes, Human, Pair 2
MH  - *Chromosomes, Human, Pair 6
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Ki-1 Antigen/*metabolism
MH  - Lymphoma, Large-Cell, Anaplastic/*genetics/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic
EDAT- 2003/07/31 05:00
MHDA- 2004/03/17 05:00
CRDT- 2003/07/31 05:00
PHST- 2003/07/31 05:00 [pubmed]
PHST- 2004/03/17 05:00 [medline]
PHST- 2003/07/31 05:00 [entrez]
AID - 10.1002/dc.10315 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2003 Aug;29(2):61-6. doi: 10.1002/dc.10315.