PMID- 12889688
OWN - NLM
STAT- MEDLINE
DCOM- 20031105
LR  - 20190906
IS  - 0393-2990 (Print)
IS  - 0393-2990 (Linking)
VI  - 18
IP  - 5
DP  - 2003
TI  - Reproducibility over time of measurements of androgens, estrogens and hydroxy 
      estrogens in urine samples from post-menopausal women.
PG  - 417-24
AB  - Sex steroid concentrations in urine samples from post-menopausal women have been 
      associated with risk of various chronic diseases. The basic requirement for the 
      assessment of risk in such large-scale epidemiological studies is that subjects 
      be ranked accurately by their average, long-term hormone levels. We examined the 
      reproducibility over time of measurements of urinary testosterone (T), 
      5alpha-androstane-3alpha, 17beta-diol (ADIOL), estrone (E1), estradiol (E2), 
      2-hydroxy estrone and 2-hydroxy estradiol, (2(OH)-E), 16alpha-hydroxyestrone 
      (16alpha(OH)-E1) and the ratio of 2(OH)-E and 16alpha(OH)-E1, in a representative 
      sub-sample of post-menopausal women (n = 43) participating in an ongoing 
      prospective cohort study. Women collected three first morning urine voids on 
      different occasions, with average time difference between the first and the third 
      urine sample of 5.1 years. T, ADIOL, E1 and E2 were measured by radio immunoassay 
      after enzymatic hydrolysis, solid-phase extraction and HPLC purification of the 
      samples, while 2(OH)-E and 16alpha(OH)-E1 were assayed by solid-phase enzyme 
      immunoassay after enzymatic hydrolysis. Intra-class correlation co-efficients 
      (ICCs) over time were very good for T (r = 0.85), acceptable for E2, E1 and ADIOL 
      (r > 0.55), but low for 2(OH)-E, 16alpha(OH)-E1 and their ratio (r < 0.46). The 
      adjustment for creatinine concentrations did not increase these correlations.
FAU - Rinaldi, S
AU  - Rinaldi S
AD  - International Agency for Research on Cancer, Hormones and Cancer Group, 69372 
      Lyon, France. rinaldi@iarc.fr
FAU - Moret, C N
AU  - Moret CN
FAU - Kaaks, R
AU  - Kaaks R
FAU - Biessy, C
AU  - Biessy C
FAU - Kurzer, M S
AU  - Kurzer MS
FAU - Dechaud, H
AU  - Dechaud H
FAU - Peeters, P H M
AU  - Peeters PH
FAU - van Noord, P A H
AU  - van Noord PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Epidemiol
JT  - European journal of epidemiology
JID - 8508062
RN  - 0 (Androgens)
RN  - 0 (Estrogens)
RN  - 0 (Hydroxyestrones)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Aged
MH  - Androgens/*urine
MH  - Breast Neoplasms/urine
MH  - Creatinine/urine
MH  - Estrogens/*urine
MH  - Female
MH  - Humans
MH  - Hydrolysis
MH  - Hydroxyestrones/urine
MH  - Immunoenzyme Techniques/methods
MH  - Middle Aged
MH  - Netherlands
MH  - Pilot Projects
MH  - Postmenopause/*urine
MH  - Reproducibility of Results
MH  - Risk Factors
MH  - Surveys and Questionnaires
MH  - Time Factors
MH  - Urinalysis/methods
EDAT- 2003/08/02 05:00
MHDA- 2003/11/06 05:00
CRDT- 2003/08/02 05:00
PHST- 2003/08/02 05:00 [pubmed]
PHST- 2003/11/06 05:00 [medline]
PHST- 2003/08/02 05:00 [entrez]
AID - 10.1023/a:1024209122412 [doi]
PST - ppublish
SO  - Eur J Epidemiol. 2003;18(5):417-24. doi: 10.1023/a:1024209122412.