PMID- 12889726
OWN - NLM
STAT- MEDLINE
DCOM- 20031209
LR  - 20181113
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 67
IP  - 3
DP  - 2003 Jul
TI  - Survival of porcine reproductive and respiratory syndrome virus in houseflies.
PG  - 198-203
AB  - The objectives of the study were to determine the duration of porcine 
      reproductive and respiratory syndrome virus (PRRSV) survival in houseflies (Musca 
      domestica Linnaeus) following feeding on an infected pig, and to determine 
      whether the virus was present on the exterior surface or within the internal 
      viscera of the fly. A total of 210 laboratory-colonized houseflies were allowed 
      to feed to repletion on a pig, experimentally infected with PRRSV on day 7 
      postinoculation, and then maintained alive under laboratory conditions (27 
      degrees C). Two subsets (A and B) of 30 flies were collected at each of the 
      following sampling points; 0, 6, and 12 hours post feeding (pf). Subset A 
      contained an extra group of 30 flies collected at 24 hours pf due to the 
      availability of extra flies. Flies in subset A were processed as whole fly 
      homogenates, while the exterior surface washes and digestive organs were 
      collected from flies in subset B. Whole fly homogenates, collected at 0, 6, and 
      12 hours pf, were positive by both polymerase chain reaction (PCR) and swine 
      bioassay. Digestive organs, collected at 0 and 12 hours pf, were positive by PCR 
      and swine bioassay. The PRRSV RNA was detected by PCR from the exterior surface 
      wash of subset B flies collected at 0, 6, and 12 hours pf; however, only the 
      subset collected at 0 hour pf was swine bioassay-positive. This study indicates 
      that infectious PRRSV can survive within the intestinal tract of houseflies for 
      up to 12 hours following feeding on an infected pig, but only for a short period 
      on the exterior surface of the flies.
FAU - Otake, Satoshi
AU  - Otake S
AD  - Swine Disease Eradication Center, University of Minnesota College of Veterinary 
      Medicine, University of Minnesota, 1988 Fitch Avenue, St. Paul, Minnesota 55108, 
      USA. otak0001@tc.umn.edu
FAU - Dee, Scott A
AU  - Dee SA
FAU - Moon, Roger D
AU  - Moon RD
FAU - Rossow, Kurt D
AU  - Rossow KD
FAU - Trincado, Carlos
AU  - Trincado C
FAU - Farnham, MacDonald
AU  - Farnham M
FAU - Pijoan, Carlos
AU  - Pijoan C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Biological Assay/veterinary
MH  - Houseflies/*virology
MH  - Insect Vectors/*virology
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/blood/*transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*growth & 
      development
MH  - RNA, Viral/isolation & purification
MH  - Swine
MH  - Time Factors
PMC - PMC227053
EDAT- 2003/08/02 05:00
MHDA- 2003/12/11 05:00
PMCR- 2003/10/01
CRDT- 2003/08/02 05:00
PHST- 2003/08/02 05:00 [pubmed]
PHST- 2003/12/11 05:00 [medline]
PHST- 2003/08/02 05:00 [entrez]
PHST- 2003/10/01 00:00 [pmc-release]
AID - 0000000-200307000-00007 [pii]
PST - ppublish
SO  - Can J Vet Res. 2003 Jul;67(3):198-203.