PMID- 12890388
OWN - NLM
STAT- MEDLINE
DCOM- 20031017
LR  - 20221207
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 116
IP  - 7
DP  - 2003 Jul
TI  - Association between HLA-DQA1, -DQB1 gene polymorphisms and susceptibility to 
      asthma in northern Chinese subjects.
PG  - 1078-82
AB  - OBJECTIVE: Human leukocyte antigen (HLA) class II genes, especially HLA-DQ genes, 
      which are highly polymorphic, have been thought to be candidate loci for the 
      etiology of asthma, and shown to be involved in antigenic presentation. This 
      study was conducted to investigate whether susceptibility or resistance to asthma 
      is associated with HLA-DQA1 and DQB1 genes polymorphism. METHODS: Venous blood 
      samples were collected from northern Chinese population with Han ethnic. (1) One 
      hundred and twenty-five unrelated asthmatic individuals and 52 subjects from 12 
      asthmatic pedigrees. (2) Ninety-six healthy controls without asthma and atopy 
      with the same ethnic. Genomic DNA was extracted using standard phenol-chloroform 
      method. The second exon of HLA-DQA1 and DQB1 genes were amplified by 
      sequence-specific primer-polymerase chain reaction (SSP-PCR) method. All 
      asthmatics had their serum IgE (total and specific) antibody or skin-prick test 
      measured, bronchial reactivity to methacholine (Mch) and bronchial reversibility 
      by beta(2)-agonist evaluated. RESULTS: HLA-DQA1 * 0104 allele and HLA-DQB1 * 0201 
      allele were significantly higher in asthmatics than those in healthy controls 
      (0.408 vs 0.177, P < 0.01; 0.568 vs 0.198, P < 0.01). Odds ratios (ORs) were 
      3.203 (95% CI 1.699 - 6.037), 5.328 (95% CI 2.883 - 9.849) respectively. 
      Conversely, HLA-DQA1 * 0301 allele and HLA-DQB1 * 0301 were significantly 
      decreased in asthmatics compared to healthy controls (0.296 vs 0.50, P < 0.01; 
      0.4 vs 0.563, P < 0.05); Logistic regression analysis showed that HLA-DQA1 * 0104 
      allele was associated independently with asthma etiology, OR [represented by 
      Exp(B)] was 5.0942 with 95% CI 2.2520 - 21.1813; Spearman's analysis showed that 
      HLA-DQA1 * 0104 allele and HLA-DQB1 * 0201 allele were positively associated with 
      atopy, the correlation coefficient were 0.183 and 0.289 respectively, P < 0.01. 
      By contrast, HLA-DQA1 * 0301 allele was negatively related to atopy, the 
      correlation coefficient was -0.168, P < 0.05; linkage analysis did not support 
      the view that HLA-DQA1/DQB1 genes were linked to asthma with LOD value being 
      0.72. CONCLUSIONS: HLA-DQA1 * 0104 allele and HLA-DQB1 * 0201 allele were 
      implicated in susceptibility to asthma and atopy, HLA-DQA1 * 0301 allele and 
      HLA-DQB1 * 0301 might be protective factor against asthma. Asthma and atopy are 
      multifactorial disorders, HLA-DQA1 and DQB1 genes are involved in the regulation 
      of immune specific response to common allergen.
FAU - Gao, Jinming
AU  - Gao J
AD  - Department of Respiratory Disease, Peking Union Medical College Hospital, Peking 
      Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, 
      China. jinming.gao@TCH.harvard.edu
FAU - Lin, Yaoguang
AU  - Lin Y
FAU - Qiu, Changchun
AU  - Qiu C
FAU - Liu, Yiwen
AU  - Liu Y
FAU - Ma, Yi
AU  - Ma Y
FAU - Liu, Ying
AU  - Liu Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQA1 antigen)
RN  - 0 (HLA-DQB1 antigen)
SB  - IM
MH  - Adult
MH  - Asian People/*genetics
MH  - Asthma/*genetics
MH  - China
MH  - *Disease Susceptibility
MH  - Female
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DQ alpha-Chains
MH  - HLA-DQ beta-Chains
MH  - Humans
MH  - Male
MH  - *Polymorphism, Genetic
EDAT- 2003/08/02 05:00
MHDA- 2003/10/18 05:00
CRDT- 2003/08/02 05:00
PHST- 2003/08/02 05:00 [pubmed]
PHST- 2003/10/18 05:00 [medline]
PHST- 2003/08/02 05:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2003 Jul;116(7):1078-82.