PMID- 12892593
OWN - NLM
STAT- MEDLINE
DCOM- 20081222
LR  - 20030801
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 41
IP  - 5
DP  - 2003 May
TI  - [Lung squamous cell carcinoma-related genes cloned by using suppression 
      subtractive hybridization].
PG  - 368-71
AB  - OBJECTIVE: To identify genes differentially expressed in human lung squamous cell 
      carcinoma (LSCC). METHODS: A subtracted cDNA library of human LSCC constructed by 
      using suppression subtracted hybridization (SSH) method was screened. Clones 
      representing mRNAs that were truly differentially expressed in LSCC but not in 
      its adjacent non-cancerous tissues were identified by semi-quantitative RT-PCR in 
      12 patients with LSCC. Partial novel genes were detected by Northern blot. 
      RESULTS: Ten differentially expressed gene cDNA fragments of LSSC were obtained 
      by SSH. Among them six were known genes; two sequences were identified but their 
      functions were unknown (hypothetical protein); two were novel (GenBank accession 
      numbers AF363068 and AY032661, respectively). The results from semiquantitative 
      RT-PCR showed that the transcription expression level of PPP1CB, calumenin, 
      S100A2, HSNOV1, OCIA and AY032661 was down-regulated in some LSCC cases, while 
      the transcription of HSP90, ferritin, gp96 and AF363068 was up-regulated in 
      others. CONCLUSIONS: Six known genes identified by SSH technique have been 
      implicated in the pathogenesis of lung carcinogenesis, or they are involved in 
      immunological defense mechanism in human body. Two hypothetical proteins probably 
      also play an important role in the pathogenesis of lung cancer. The function of 
      the two novel genes in lung carcinogenesis are under investigation.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of Thoracic Surgery, People's Hospital, Peking University, Beijing 
      100044, China.
FAU - Shen, Chen-yang
AU  - Shen CY
FAU - Zhao, Hui
AU  - Zhao H
FAU - Wang, Dan-lei
AU  - Wang DL
FAU - Zhang, Guo-liang
AU  - Zhang GL
FAU - Wang, Jun
AU  - Wang J
LA  - chi
SI  - GENBANK/AF363068
SI  - GENBANK/AY032661
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Library
MH  - Humans
MH  - Lung Neoplasms/*genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization/methods
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/08/02 05:00
MHDA- 2008/12/23 09:00
CRDT- 2003/08/02 05:00
PHST- 2003/08/02 05:00 [pubmed]
PHST- 2008/12/23 09:00 [medline]
PHST- 2003/08/02 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 2003 May;41(5):368-71.