PMID- 12892735
OWN - NLM
STAT- MEDLINE
DCOM- 20040406
LR  - 20231213
IS  - 0896-8411 (Print)
IS  - 0896-8411 (Linking)
VI  - 21
IP  - 1
DP  - 2003 Aug
TI  - Linear epitopes of two different autoantigens-La/SSB and myelin basic 
      protein--with a high degree of molecular similarity, cause different humoral 
      immune responses.
PG  - 47-57
AB  - The region 147-154aa of La/SSB presents 83% sequence similarity with the 
      139-146aa region of the human myelin basic protein (MBP). The aim of this study 
      was to investigate the prevalence and significance of antibodies against both 
      epitopes in sera from patients with systemic autoimmune diseases, and to compare 
      the humoral responses produced after rabbit immunization. Peptides 147-154aa of 
      La/SSB and 139-146aa of the MBP were attached on tetrameric sequential 
      oligopeptide carriers and used for immunizations of New Zealand White rabbits. 
      Antibodies to immunizing peptides, as well as to the peptides corresponding to 
      other previously defined La/SSB epitopes (289-308aa, 349-364aa), to the intact 
      human MBP (hMBP) and to the recombinant human La/SSB (rechLa) proteins, were 
      identified using specific ELISA assays. Sera from 45 patients with Sjogren's 
      syndrome (pSS), 49 with Systemic Lupus Erythematosus (SLE), and 18 with 
      Rheumatoid Arthritis (RA) were tested against the two peptides and the hMBP. 
      Twenty-two per cent of sera from pSS patients, 27% of SLE patients, and none from 
      RA sera reacted with the La epitope; 27% from pSS sera, 22% of SLE sera, and 17% 
      of RA sera gave a positive reaction against the MBP peptide. Finally, 19% of pSS, 
      30% of SLE, and 38% of RA sera reacted with the hMBP. Thirty-five days after 
      immunization of rabbits with the La epitope, antibodies were produced against all 
      three La/SSB peptides, the MBP peptide, and the hMBP and rechLa proteins. Rabbits 
      immunized with the MBP peptide produced antibodies against the immunizing peptide 
      and the mimicking peptide of La shortly after immunization, whilst antibodies 
      against the other La epitopes and the two intact proteins were produced later. 
      Inhibition experiments in rabbit sera with high reactivity against hMBP, using 
      the MBP peptide as inhibitor, revealed that 80% of serum reactivity was 
      abolished. In conclusion, a significant proportion of human autoimmune sera 
      reacted with both La and MBP derived peptides, as well as with hMBP. La 147-154aa 
      peptide, when used for animal immunizations, induces a fast epitope spreading 
      involving both La and MBP. In contrast, the mimicking MBP epitope induces a 
      delayed response against the other La epitopes. Thus, despite the fact that these 
      peptides present molecular similarity, they induce different immune responses.
FAU - Terzoglou, Apostolos G
AU  - Terzoglou AG
AD  - Department of Pathophysiology, School of Medicine, University of Athens, 75 M. 
      Asias st, 11527 Athens, Greece.
FAU - Routsias, John G
AU  - Routsias JG
FAU - Sakarellos, Constantinos
AU  - Sakarellos C
FAU - Sakarellos-Daitsiotis, Maria
AU  - Sakarellos-Daitsiotis M
FAU - Moutsopoulos, Haralampos M
AU  - Moutsopoulos HM
FAU - Tzioufas, Athanasios G
AU  - Tzioufas AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Autoimmun
JT  - Journal of autoimmunity
JID - 8812164
RN  - 0 (Autoantigens)
RN  - 0 (Epitopes)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (Peptides)
RN  - 0 (Ribonucleoproteins)
SB  - IM
MH  - Animals
MH  - Antibody Formation/*immunology
MH  - Autoantigens
MH  - Autoimmune Diseases/immunology
MH  - Epitopes/*immunology
MH  - Humans
MH  - Kinetics
MH  - Myelin Basic Protein/*immunology
MH  - Peptides/*immunology
MH  - Rabbits
MH  - Ribonucleoproteins/*immunology
MH  - SS-B Antigen
EDAT- 2003/08/02 05:00
MHDA- 2004/04/07 05:00
CRDT- 2003/08/02 05:00
PHST- 2003/08/02 05:00 [pubmed]
PHST- 2004/04/07 05:00 [medline]
PHST- 2003/08/02 05:00 [entrez]
AID - S0896841103000829 [pii]
AID - 10.1016/s0896-8411(03)00082-9 [doi]
PST - ppublish
SO  - J Autoimmun. 2003 Aug;21(1):47-57. doi: 10.1016/s0896-8411(03)00082-9.