PMID- 12893257
OWN - NLM
STAT- MEDLINE
DCOM- 20030904
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 307
IP  - 3
DP  - 2003 Aug 1
TI  - Oxidation of glyceraldehyde-3-phosphate dehydrogenase enhances its binding to 
      nucleic acids.
PG  - 547-52
AB  - Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a protein with various 
      activities far from its enzymatic function. Here, we showed that the oxidation of 
      SH-groups of the active site of GAPDH enhanced its binding with total transfer 
      RNA or with total DNA. Both NAD and NADH-the cofactors of GAPDH-inhibited the 
      GAPDH-RNA (DNA) interaction, though NAD was much less effective than NADH in the 
      case of oxidized GAPDH. Oxidation of GAPDH strongly decreased its affinity to NAD 
      but not to NADH. Immobilized tetramers of GAPDH dissociated into dimers during 
      the incubation with total RNA but not DNA. The staining of HeLa cells with 
      monoclonal antibodies specific to dimers, monomers or the denatured form of GAPDH 
      revealed the condensation of non-native forms of GAPDH in the nucleus. The role 
      of oxidation of GAPDH in the regulation of the quaternary structure of the enzyme 
      and in its interaction with nucleic acids is discussed.
FAU - Arutyunova, Elena I
AU  - Arutyunova EI
AD  - Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 
      19992, Russia.
FAU - Danshina, Pauline V
AU  - Danshina PV
FAU - Domnina, Lydia V
AU  - Domnina LV
FAU - Pleten, Anatoliy P
AU  - Pleten AP
FAU - Muronetz, Vladimir I
AU  - Muronetz VI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0U46U6E8UK (NAD)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
RN  - EC 1.2.1.12 (Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating))
SB  - IM
MH  - Binding Sites
MH  - Cell Nucleus/chemistry
MH  - DNA/*metabolism
MH  - Glyceraldehyde-3-Phosphate Dehydrogenase 
      (Phosphorylating)/analysis/chemistry/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Microscopy, Fluorescence
MH  - NAD/metabolism
MH  - Oxidation-Reduction
MH  - Protein Binding
MH  - Protein Structure, Quaternary
MH  - RNA/*metabolism
EDAT- 2003/08/02 05:00
MHDA- 2003/09/05 05:00
CRDT- 2003/08/02 05:00
PHST- 2003/08/02 05:00 [pubmed]
PHST- 2003/09/05 05:00 [medline]
PHST- 2003/08/02 05:00 [entrez]
AID - S0006291X03012221 [pii]
AID - 10.1016/s0006-291x(03)01222-1 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2003 Aug 1;307(3):547-52. doi: 
      10.1016/s0006-291x(03)01222-1.