PMID- 12894496
OWN - NLM
STAT- MEDLINE
DCOM- 20030828
LR  - 20071115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 23
IP  - 3B
DP  - 2003 May-Jun
TI  - In situ RNA-RNA hybridisation of phospholipase C beta 3 shows lack of expression 
      in neuroendocrine tumours.
PG  - 2227-32
AB  - BACKGROUND: Phospholipase C beta 3 (PLCB3) plays an important role in the signal 
      transduction of the seven transmembrane receptors. The gene is located in the 
      vicinity of the Multiple Endocrine Neoplasia type 1 (MEN1) gene on chromosome 
      11q13. Transfection of PLCB3 to neuroendocrine cell lines lacking expression 
      suppresses the neoplastic phenotype and affects the gene expression of S100A3 and 
      human mismatch repair protein, suggesting a role for PLCB3 in neuroendocrine 
      tumorigenesis. MATERIALS AND METHODS: We used RNA-RNA in situ hybridisation for 
      PLCB3 on a total of 82 samples including 34 from MEN1 patients. RESULTS: We show 
      that the PLCB3 transcript is missing in 8 out of 14 MEN1-associated neoplasias as 
      well as in 4 out of 10 bronchial carcinoids, 2 out of 10 exocrine pancreatic 
      cancers and one sporadic adrenocortical carcinoma. CONCLUSION: Low or lack of 
      PLCB3 expression in a subset of endocrine tumours, together with earlier 
      published in vitro data on suppressor characteristics upon transfection, indicate 
      that PLCB3 could be involved in the tumorigenesis in a subset of endocrine 
      tumours.
FAU - Stalberg, Peter
AU  - Stalberg P
AD  - Department of Surgical Sciences, University Hospital, S-751 85 Uppsala, Sweden. 
      Peter.Stalberg@surgsci.uu.se
FAU - Granberg, Dan
AU  - Granberg D
FAU - Carling, Tobias
AU  - Carling T
FAU - Wilander, Erik
AU  - Wilander E
FAU - Eriksson, Barbro
AU  - Eriksson B
FAU - Gobl, Anders
AU  - Gobl A
FAU - Akerstrom, Goran
AU  - Akerstrom G
FAU - Rastad, Jonas
AU  - Rastad J
FAU - Modlin, Irvin M
AU  - Modlin IM
FAU - Oberg, Kjell
AU  - Oberg K
FAU - Skogseid, Britt
AU  - Skogseid B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Neoplasm)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.11 (PLCB3 protein, human)
RN  - EC 3.1.4.11 (Phospholipase C beta)
SB  - IM
MH  - Animals
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Isoenzymes/biosynthesis/*genetics
MH  - Multiple Endocrine Neoplasia Type 1/*enzymology/genetics
MH  - Muridae
MH  - Neuroendocrine Tumors/*enzymology/genetics
MH  - Phospholipase C beta
MH  - RNA, Neoplasm/*biosynthesis/genetics
MH  - Type C Phospholipases/biosynthesis/*genetics
EDAT- 2003/08/05 05:00
MHDA- 2003/08/29 05:00
CRDT- 2003/08/05 05:00
PHST- 2003/08/05 05:00 [pubmed]
PHST- 2003/08/29 05:00 [medline]
PHST- 2003/08/05 05:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2003 May-Jun;23(3B):2227-32.