PMID- 12894528
OWN - NLM
STAT- MEDLINE
DCOM- 20030828
LR  - 20171116
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 23
IP  - 3B
DP  - 2003 May-Jun
TI  - Dendritic cell maturation induced by delivery of ultraviolet-mediated apoptotic 
      colorectal cancer cell lines.
PG  - 2457-63
AB  - BACKGROUND: Dendritic cells (DCs) play a critical role in the immune response. 
      The aim of this study was to investigate apoptotic tumor cells as an antigen 
      source for DC maturation. MATERIALS AND METHODS: We compared the efficacy of 
      ultraviolet (UV)-irradiation with that of gamma-irradiation in the induction of 
      apoptosis of tumor cells. Phenotypic and functional changes of DCs were analyzed 
      after co-incubation with UV-irradiated tumor cells. RESULTS: UV-irradiation (1.8 
      J/cm2) was a more reliable method of inducing apoptosis than gamma-irradiation 
      (20,000 rad). The expression of costimulatory molecules on DCs was upregulated 
      after co-incubation with UV-irradiated tumor cells. When we performed allogeneic 
      mixed lymphocyte reaction assay, UV-irradiated tumor cells-pulsed DCs stimulated 
      allogeneic T lymphocytes more efficiently than DCs pulsed with gamma-irradiated 
      cells (HT29, p = 0.0419 and WiDr, p = 0.0076). CONCLUSION: UV-irradiation 
      reliably induces apoptosis in cultured colorectal cancer cells. DCs mature in 
      function and expression of costimulatory molecules after co-incubation with 
      apoptotic tumor cells. The clinical implications warrant further study.
FAU - Ishii, Shigeaki
AU  - Ishii S
AD  - Second Department of Internal Medicine, Showa University School of Medicine, 
      1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.
FAU - Hiroishi, Kazumasa
AU  - Hiroishi K
FAU - Eguchi, Jun-Ichi
AU  - Eguchi J
FAU - Mitamura, Keiji
AU  - Mitamura K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Antigens, CD/biosynthesis/immunology
MH  - Apoptosis/radiation effects
MH  - B7-1 Antigen/biosynthesis/immunology
MH  - B7-2 Antigen
MH  - Cell Communication/immunology
MH  - Colorectal Neoplasms/*immunology/pathology
MH  - Dendritic Cells/cytology/*immunology
MH  - HT29 Cells
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - Membrane Glycoproteins/biosynthesis/immunology
MH  - Tumor Cells, Cultured
MH  - Ultraviolet Rays
MH  - Up-Regulation
EDAT- 2003/08/05 05:00
MHDA- 2003/08/29 05:00
CRDT- 2003/08/05 05:00
PHST- 2003/08/05 05:00 [pubmed]
PHST- 2003/08/29 05:00 [medline]
PHST- 2003/08/05 05:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2003 May-Jun;23(3B):2457-63.