PMID- 12894841
OWN - NLM
STAT- MEDLINE
DCOM- 20031007
LR  - 20190823
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 128
IP  - 7
DP  - 2003 Jul
TI  - Study on Schiff base complexes-cellular DNA interactions by a novel system of 
      Hadamard transform fluorescence image microscopy.
PG  - 974-9
AB  - A novel system of Hadamard transform microscopic fluorescence imaging for single 
      cells is presented, based on which the DNA ploidy of rat hepatocyte was 
      quantitatively measured. The result shows that diploid rat hepatocyte has a 
      stable DNA content, thus diploid rat hepatocyte was used to investigate the 
      binding of five clinical anticancer agents, vincristine, cyclophosphamide, 
      nitrogen mustard, cis-diamminedichloroplatinum(n) (CDDP) and mitomycin-C, with 
      cellular DNA when acridine orange (AO) was used as the competitive fluorescence 
      probe. Based on this model, some Schiff base complexes-cellular DNA interactions 
      were investigated. The results indicate that all the twenty-two compounds, 
      including Schiff base ligands of N-2-hydroxy-naphthaldehyde with D-glucoamine 
      (NG) and the complexes of 3d-transitional metals ions with NO and with 
      D-glucoamine (Glu) and the mixed complexes of NG and Glu series with 
      alpha-glycine (GNG), have the ability to enter the cell membrane and interact 
      with cellular DNA. Four of the compounds, CuGlu, Fe(II)NG, Fe(III)NG and CuGluG 
      can intercalate with DNA like AO does and depress AO-DNA fluorescence to 70% or 
      lower. An in intro UV-visible spectroscopic study on the compound-DNA spectra 
      testified the above results and suggests that diverse interaction mechanisms 
      coexist for all these complexes except intercalating mode. This study presents a 
      new in vitro method for initial screening of anticancer compounds.
FAU - Tang, Hongwu
AU  - Tang H
AD  - Centre of Analysis and Measurements, College of Chemistry and Molecular Science, 
      Wuhan University, Wuhan 430072, P. R. China. hwtang@whu.edu.cn
FAU - Ye, Yong
AU  - Ye Y
FAU - Li, Tao
AU  - Li T
FAU - Zhou, Jinsong
AU  - Zhou J
FAU - Chen, Guanquan
AU  - Chen G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Schiff Bases)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*metabolism
MH  - DNA/*metabolism
MH  - Drug Screening Assays, Antitumor/*methods
MH  - Hepatocytes/metabolism
MH  - In Vitro Techniques
MH  - Microscopy, Fluorescence/methods
MH  - Ploidies
MH  - Rats
MH  - Schiff Bases/*metabolism
EDAT- 2003/08/05 05:00
MHDA- 2003/10/08 05:00
CRDT- 2003/08/05 05:00
PHST- 2003/08/05 05:00 [pubmed]
PHST- 2003/10/08 05:00 [medline]
PHST- 2003/08/05 05:00 [entrez]
AID - 10.1039/b300489a [doi]
PST - ppublish
SO  - Analyst. 2003 Jul;128(7):974-9. doi: 10.1039/b300489a.