PMID- 12895416
OWN - NLM
STAT- MEDLINE
DCOM- 20030902
LR  - 20220309
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 39
IP  - 3
DP  - 2003 Jul 31
TI  - Corticotropin-releasing factor requires CRF binding protein to potentiate NMDA 
      receptors via CRF receptor 2 in dopamine neurons.
PG  - 401-7
AB  - Stress increases addictive behaviors and is a common cause of relapse. 
      Corticotropin-releasing factor (CRF) plays a key role in the modulation of drug 
      taking by stress. However, the mechanism by which CRF modulates neuronal activity 
      in circuits involved in drug addiction is poorly understood. Here we show that 
      CRF induces a potentiation of NMDAR (N-methyl-D-aspartate receptor)-mediated 
      synaptic transmission in dopamine neurons of the ventral tegmental area (VTA). 
      This effect involves CRF receptor 2 (CRF-R2) and activation of the phospholipase 
      C (PLC)-protein kinase C (PKC) pathway. We also find that this potentiation 
      requires CRF binding protein (CRF-BP). Accordingly, CRF-like peptides, which do 
      not bind the CRF-BP with high affinity, do not potentiate NMDARs. These results 
      provide evidence of the first specific roles for CRF-R2 and CRF-BP in the 
      modulation of neuronal activity and suggest that NMDARs in the VTA may be a 
      target for both drugs of abuse and stress.
FAU - Ungless, Mark A
AU  - Ungless MA
AD  - Ernest Gallo Clinic and Research Center and Department of Neurology, University 
      of California, San Francisco, San Francisco, California 94110, USA.
FAU - Singh, Vineeta
AU  - Singh V
FAU - Crowder, Tara L
AU  - Crowder TL
FAU - Yaka, Rami
AU  - Yaka R
FAU - Ron, Dorit
AU  - Ron D
FAU - Bonci, Antonello
AU  - Bonci A
LA  - eng
GR  - K23 NS42072/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
RN  - 0 (CRF receptor type 2)
RN  - 0 (Carrier Proteins)
RN  - 0 (Receptors, Corticotropin-Releasing Hormone)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 134773-81-2 (corticotropin releasing factor-binding protein)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
CIN - Neuron. 2003 Jul 31;39(3):387-8. PMID: 12895412
MH  - Animals
MH  - Carrier Proteins/*physiology
MH  - Corticotropin-Releasing Hormone/pharmacology/*physiology
MH  - Dopamine/*metabolism
MH  - In Vitro Techniques
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/drug effects/*metabolism
MH  - Patch-Clamp Techniques
MH  - Receptors, Corticotropin-Releasing Hormone/*physiology
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
EDAT- 2003/08/05 05:00
MHDA- 2003/09/03 05:00
CRDT- 2003/08/05 05:00
PHST- 2003/08/05 05:00 [pubmed]
PHST- 2003/09/03 05:00 [medline]
PHST- 2003/08/05 05:00 [entrez]
AID - S0896627303004616 [pii]
AID - 10.1016/s0896-6273(03)00461-6 [doi]
PST - ppublish
SO  - Neuron. 2003 Jul 31;39(3):401-7. doi: 10.1016/s0896-6273(03)00461-6.