PMID- 12898274
OWN - NLM
STAT- MEDLINE
DCOM- 20040610
LR  - 20181113
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 120
IP  - 3
DP  - 2003 Sep
TI  - Detection of HER2/neu gene amplification in archival paraffin-embedded breast 
      cancer tissues by fluorescence in situ hybridization.
PG  - 251-5
AB  - We evaluated HER2/neu gene amplification by fluorescence in situ hybridization 
      (FISH) in archival paraffin-embedded breast cancer tissues. Tumors from 63 human 
      invasive breast cancers were categorized into two groups depending on whether the 
      paraffin-embedded tissue blocks had been stored for more or less than 12 months 
      duration. These were subjected to routine and modified FISH protocols. As 
      microwave oven formalin fixation of tissues was carried out in the majority of 
      the older archived specimens, the effect of this fixation method was also 
      analyzed. FISH signals were obtained in all 13 archival specimens stored for less 
      than 12 months. However, in 50 specimens stored for more than 12 months duration, 
      the procedure was successful in only 10 specimens (20%), for which the 
      pretreatment procedure had to be individually optimized for each specimen. There 
      was no significant difference in the detection of FISH signals between microwave 
      oven and routinely fixed specimens.
FAU - Selvarajan, Sathiyamoorthy
AU  - Selvarajan S
AD  - Department of Anatomy, National University of Singapore, Singapore.
FAU - Bay, Boon-Huat
AU  - Bay BH
FAU - Mamat, Shalawati Binte
AU  - Mamat SB
FAU - Choo, Andrew
AU  - Choo A
FAU - Chuah, Khoon-Leong
AU  - Chuah KL
FAU - Sivaswaren, Christina Rudduck
AU  - Sivaswaren CR
FAU - Tien, Sim-Leng
AU  - Tien SL
FAU - Wong, Chow-Yin
AU  - Wong CY
FAU - Tan, Puay-Hoon
AU  - Tan PH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20030724
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
SB  - IM
MH  - Breast Neoplasms/*genetics
MH  - Female
MH  - Genes, erbB-2/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding
EDAT- 2003/08/05 05:00
MHDA- 2004/06/21 10:00
CRDT- 2003/08/05 05:00
PHST- 2003/06/25 00:00 [accepted]
PHST- 2003/08/05 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2003/08/05 05:00 [entrez]
AID - 10.1007/s00418-003-0554-z [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2003 Sep;120(3):251-5. doi: 10.1007/s00418-003-0554-z. Epub 
      2003 Jul 24.