PMID- 12898459
OWN - NLM
STAT- MEDLINE
DCOM- 20030925
LR  - 20190725
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 52
IP  - 8
DP  - 2003 Aug
TI  - Synergistic effects of testosterone and growth hormone on protein metabolism and 
      body composition in prepubertal boys.
PG  - 964-9
AB  - During human puberty there is a substantial increase in growth hormone (GH) and 
      sex steroidal hormone concentrations, as well as in GH production rates and 
      insulin-like growth factor-I (IGF-I) These studies were designed to investigate 
      some of the interactions of testosterone (T) and GH in the metabolic changes of 
      puberty. Ten boys with severe GH deficiency (GHD) were studied (mean age, 12.5 
      +/- 0.5 years) using stable isotope infusions, indirect calorimetry, and body 
      composition analysis. After the baseline study, they received 2 doses of T 
      enanthate (50 to 75 mg, intramuscular [IM]), and they were studied again 4 weeks 
      later. The boys were then begun on daily subcutaneous (SC) GH (0.3 mg/kg/wk), 
      while T therapy was continued for another 4 weeks and the studies repeated a 
      third time. The treatment order was randomized. Protein oxidation rates decreased 
      after T alone (-28%, P <.01), decreasing further after combined T/GH treatment 
      (-36% v baseline, P <.01). The nonoxidative leucine disposal (NOLD), a measure of 
      whole body protein synthesis, increased significantly after combined T/GH 
      regardless of treatment order. The combination of T/GH also resulted in greater 
      changes in body composition than T alone, with comparable decreases in %FM and 
      corresponding increases in fat free mass (FFM). Measures of carbohydrate (CHO) 
      metabolism, including glucose production and oxidation rates, were unaffected by 
      either T or T/GH combination. Plasma IGF-I concentrations increased after T 
      treatment and even more after T/GH combination, regardless of the treatment 
      order. In conclusion GH and T are synergistic on whole body protein anabolism and 
      body composition in males, even at a young age, but the positive effects of T on 
      protein anabolism and body composition appear to need a basal amount of GH for 
      those effects to be observed. GH and T both potentiate the development of the 
      full body composition and metabolic changes of puberty.
FAU - Mauras, Nelly
AU  - Mauras N
AD  - Nemours Children's Clinic Division of Endocrinology and Nemours Research Program, 
      Jacksonville, FL 32207, USA.
FAU - Rini, Annie
AU  - Rini A
FAU - Welch, Susan
AU  - Welch S
FAU - Sager, Brenda
AU  - Sager B
FAU - Murphy, Suzanne P
AU  - Murphy SP
LA  - eng
GR  - R01DK51360/DK/NIDDK NIH HHS/United States
GR  - RR-00585/RR/NCRR NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Growth Substances)
RN  - 0 (Hormones)
RN  - 0 (Keto Acids)
RN  - 0 (Proteins)
RN  - 3XMK78S47O (Testosterone)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 816-66-0 (alpha-ketoisocaproic acid)
RN  - 9002-72-6 (Growth Hormone)
RN  - GMW67QNF9C (Leucine)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Body Composition/*drug effects
MH  - Carbohydrate Metabolism
MH  - Child
MH  - Drug Synergism
MH  - Energy Metabolism/physiology
MH  - Glucose/metabolism
MH  - Growth Hormone/*pharmacology
MH  - Growth Substances/blood
MH  - Hormones/blood
MH  - Humans
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Keto Acids/metabolism
MH  - Leucine/metabolism
MH  - Male
MH  - Oxidation-Reduction
MH  - Proteins/*metabolism
MH  - Sexual Maturation/physiology
MH  - Testosterone/*pharmacology
EDAT- 2003/08/05 05:00
MHDA- 2003/09/26 05:00
CRDT- 2003/08/05 05:00
PHST- 2003/08/05 05:00 [pubmed]
PHST- 2003/09/26 05:00 [medline]
PHST- 2003/08/05 05:00 [entrez]
AID - S002604950300163X [pii]
AID - 10.1016/s0026-0495(03)00163-x [doi]
PST - ppublish
SO  - Metabolism. 2003 Aug;52(8):964-9. doi: 10.1016/s0026-0495(03)00163-x.