PMID- 12898595
OWN - NLM
STAT- MEDLINE
DCOM- 20031006
LR  - 20091119
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 200
IP  - 5
DP  - 2003 Aug
TI  - Immunoreactivity for cyclin D3 is frequently detectable in high-grade primary 
      gastric lymphomas in the absence of the t(6;14)(p21.1;q32.3) chromosomal 
      translocation.
PG  - 596-601
AB  - Cyclin D3 plays a pivotal role in controlling the physiological progression from 
      the G1 to the S phase of the cell cycle. Recent data suggest that cyclin D3 may 
      be deregulated in extranodal non-Hodgkin's lymphomas (NHLs) as a consequence of 
      the t(6;14)(p21.1;q32.3) translocation. The present study investigated for the 
      first time by dual-colour fluorescence in situ hybridization (FISH) on interphase 
      nuclei and immunohistochemistry the prevalence of the t(6;14) translocation and 
      cyclin D3 immunoreactivity (IR) in a series of 29 stage I-IIE primary gastric 
      NHLs (PGLs). No case showed the t(6;14) translocation. However, in five (17.2%) 
      cases (two extranodal marginal zone lymphomas of MALT type, LGM; one diffuse 
      large-cell lymphoma with a MALT component, DLCLM; and two diffuse large-cell 
      lymphomas without a MALT component, DLCL), three to four cyclin D3 signals were 
      detected by FISH. Co-hybridization with probes specific for the centromeric 
      region and long arm of chromosome 6 indicated trisomy in one case (DLCL), whereas 
      in the remaining four cases the pattern was highly suggestive of the presence of 
      an isochromosome 6p. One (12.5%) case of LGM, six (75%) cases of DLCLM, and seven 
      (53.8%) cases of DLCL (p = 0.0378) were immunoreactive for cyclin D3. Cyclin D3 
      IR was detected in two (40%) of the five cases with extra cyclin D3 signals and 
      in 12 of the remaining 24 cases (50%, p = 1.000). These results suggest that the 
      t(6;14) may represent a rare event in the pathogenesis of PGL and that cyclin D3 
      deregulation is most likely the result of epigenetic mechanisms.
CI  - Copyright 2003 John Wiley & Sons, Ltd.
FAU - Pruneri, Giancarlo
AU  - Pruneri G
AD  - Divisions of Pathology and Laboratory Medicine, European Institute of Oncology, 
      Milan, Italy.
FAU - Fabris, Sonia
AU  - Fabris S
FAU - Fasani, Roberta
AU  - Fasani R
FAU - Del Curto, Barbara
AU  - Del Curto B
FAU - Capella, Carlo
AU  - Capella C
FAU - Pozzi, Barbara
AU  - Pozzi B
FAU - Motta, Teresio
AU  - Motta T
FAU - Andreola, Salvatore
AU  - Andreola S
FAU - Ferreri, Andres J M
AU  - Ferreri AJ
FAU - Ponzoni, Maurilio
AU  - Ponzoni M
FAU - Viale, Giuseppe
AU  - Viale G
FAU - Neri, Antonino
AU  - Neri A
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (CCND3 protein, human)
RN  - 0 (Cyclin D3)
RN  - 0 (Cyclins)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Chromosomes, Human, Pair 6/genetics
MH  - Cyclin D3
MH  - Cyclins/*metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/genetics/*metabolism
MH  - Lymphoma, B-Cell, Marginal Zone/genetics/metabolism
MH  - Lymphoma, Non-Hodgkin/genetics/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Proteins/*metabolism
MH  - Stomach Neoplasms/genetics/*metabolism
MH  - Translocation, Genetic
EDAT- 2003/08/05 05:00
MHDA- 2003/10/08 05:00
CRDT- 2003/08/05 05:00
PHST- 2003/08/05 05:00 [pubmed]
PHST- 2003/10/08 05:00 [medline]
PHST- 2003/08/05 05:00 [entrez]
AID - 10.1002/path.1384 [doi]
PST - ppublish
SO  - J Pathol. 2003 Aug;200(5):596-601. doi: 10.1002/path.1384.