PMID- 12899918
OWN - NLM
STAT- MEDLINE
DCOM- 20030903
LR  - 20220408
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 73
IP  - 15
DP  - 2003 Aug 29
TI  - Antihyperglycemic effect of aqueous and ethanolic extracts of Gongronema 
      latifolium leaves on glucose and glycogen metabolism in livers of normal and 
      streptozotocin-induced diabetic rats.
PG  - 1925-38
AB  - The present study was designed to investigate the antihyperglycemic effects of 
      aqueous and ethanolic extracts from Gongronema latifolium leaves on glucose and 
      glycogen metabolism in livers of non-diabetic and streptozotocin-induced diabetic 
      rats. To investigate the effects of aqueous or ethanolic leaf extracts of G. 
      latifolium, non-diabetic and STZ diabetic rats were treated twice daily (100 
      mg/Kg) for two weeks. Diabetic rats showed a significant decrease in the 
      activities of hepatic hexokinase (HK), phosphofructokinase (PFK) and 
      glucose-6-phosphate dehydrogenase (G6PDH) and an increase in glucokinase (GK) 
      activity. The levels of hepatic glycogen and glucose were also increased in 
      diabetic rats. However, there were no significant differences in the activities 
      of glucose-6-phosphatase (G6Pase) in treated and untreated diabetic rats. The 
      ethanolic extract significantly increased the activities of HK (p<0.01), PFK 
      (p<0.001) and G6PDH (p<0.01) in diabetic rats, decreased the activity of GK 
      (p<0.05) and the levels of hepatic glycogen (p<0.01) and both hepatic (p<0.001) 
      and blood glucose (40%). The aqueous extract of G. latifolium was only able to 
      significantly increase the activities of HK and decrease the activities of GK but 
      did not produce any significant change in the hepatic glycogen and both hepatic 
      and blood glucose content of diabetic rats. Our data show that the ethanolic 
      extract from G. latifolium leaves has antihyperglycemic potency, which is thought 
      to be mediated through the activation of HK, PFK, G6PDH and inhibition of GK in 
      the liver. The ethanolic extract is under further investigation to determine the 
      chemical structure of the active compound(s) and its/their mechanism of action.
FAU - Ugochukwu, N H
AU  - Ugochukwu NH
AD  - Department of Chemistry, Florida Agricultural and Mechanical University, 
      Tallahassee, FL 32307, USA. ngozi.ugochukwu@famu.edu
FAU - Babady, N E
AU  - Babady NE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Plant Extracts)
RN  - 059QF0KO0R (Water)
RN  - 3K9958V90M (Ethanol)
RN  - 5W494URQ81 (Streptozocin)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.7.2.- (Phosphotransferases (Carboxyl Group Acceptor))
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Apocynaceae/*chemistry
MH  - Diabetes Mellitus, Experimental/blood/*drug therapy
MH  - Ethanol/chemistry/pharmacology
MH  - Glucose/*metabolism
MH  - Glycogen/*metabolism
MH  - Hyperglycemia/*drug therapy
MH  - Hypoglycemic Agents/pharmacology/*therapeutic use
MH  - Liver/drug effects/*enzymology
MH  - Male
MH  - Phosphotransferases (Carboxyl Group Acceptor)
MH  - Plant Extracts/chemistry/pharmacology/*therapeutic use
MH  - Plant Leaves/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Streptozocin
MH  - Water/chemistry/pharmacology
EDAT- 2003/08/06 05:00
MHDA- 2003/09/04 05:00
CRDT- 2003/08/06 05:00
PHST- 2003/08/06 05:00 [pubmed]
PHST- 2003/09/04 05:00 [medline]
PHST- 2003/08/06 05:00 [entrez]
AID - S0024320503005435 [pii]
AID - 10.1016/s0024-3205(03)00543-5 [doi]
PST - ppublish
SO  - Life Sci. 2003 Aug 29;73(15):1925-38. doi: 10.1016/s0024-3205(03)00543-5.