PMID- 12900521
OWN - NLM
STAT- MEDLINE
DCOM- 20030922
LR  - 20181113
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 198
IP  - 3
DP  - 2003 Aug 4
TI  - The neurotrophins nerve growth factor, brain-derived neurotrophic factor, 
      neurotrophin-3, and neurotrophin-4 are survival and activation factors for 
      eosinophils in patients with allergic bronchial asthma.
PG  - 455-67
AB  - Neurotrophins (nerve growth factor [NGF], brain-derived neurotrophic factor 
      [BDNF], neurotrophin [NT]-3, and NT-4) have been observed in elevated 
      concentrations in allergic diseases. Neurotrophin levels are up-regulated 
      endobronchially after allergen challenge. This coincides with an influx of 
      activated eosinophils into the bronchial lumen. These eosinophils have an 
      increased viability and CD69 expression 18 h after segmental allergen provocation 
      (SAP) which is not present in peripheral blood. To investigate whether these 
      observations are related we studied the influence of neurotrophins on eosinophil 
      function in allergic asthma. Incubation with NGF, BDNF, NT-3, or NT-4 caused a 
      significant increase in the viability and CD69 expression of isolated eosinophils 
      from bronchoalveolar lavage fluid (BALF) but not from peripheral blood, 
      suggesting a unique sensitivity of endobronchial eosinophils to neurotrophins. To 
      elucidate the underlying mechanisms expression of the neurotrophin receptors 
      p75NTR, trkA, trkB, and trkC on eosinophils was analyzed by RT-PCR and 
      immunocytology. After SAP expression of all neurotrophin receptors was markedly 
      elevated on eosinophils from BALF. Our findings suggest that 
      neurotrophin-mediated activation of bronchial eosinophils might play a role in 
      the regulation of eosinophilic inflammation in allergic asthma.
FAU - Nassenstein, Christina
AU  - Nassenstein C
AD  - Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany.
FAU - Braun, Armin
AU  - Braun A
FAU - Erpenbeck, Veit Johannes
AU  - Erpenbeck VJ
FAU - Lommatzsch, Marek
AU  - Lommatzsch M
FAU - Schmidt, Stephanie
AU  - Schmidt S
FAU - Krug, Norbert
AU  - Krug N
FAU - Luttmann, Werner
AU  - Luttmann W
FAU - Renz, Harald
AU  - Renz H
FAU - Virchow, Johann Christian Jr
AU  - Virchow JC Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (CD69 antigen)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.10.1 (Receptor, trkC)
RN  - P658DCA9XD (neurotrophin 4)
SB  - IM
MH  - Adult
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, T-Lymphocyte/metabolism
MH  - Asthma/immunology/metabolism/*physiopathology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - *Bronchial Hyperreactivity/immunology
MH  - Bronchial Provocation Tests
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Eosinophils/cytology/*metabolism
MH  - Female
MH  - Forced Expiratory Volume
MH  - Humans
MH  - Lectins, C-Type
MH  - Male
MH  - Nerve Growth Factor/*metabolism
MH  - Nerve Growth Factors/*metabolism
MH  - Neuroprotective Agents/metabolism
MH  - Neurotrophin 3/*metabolism
MH  - Receptor, Nerve Growth Factor
MH  - Receptor, trkA/genetics/metabolism
MH  - Receptor, trkB/genetics/metabolism
MH  - Receptor, trkC/genetics/metabolism
MH  - Receptors, Nerve Growth Factor/genetics/metabolism
PMC - PMC2194097
EDAT- 2003/08/06 05:00
MHDA- 2003/09/23 05:00
PMCR- 2004/02/04
CRDT- 2003/08/06 05:00
PHST- 2003/08/06 05:00 [pubmed]
PHST- 2003/09/23 05:00 [medline]
PHST- 2003/08/06 05:00 [entrez]
PHST- 2004/02/04 00:00 [pmc-release]
AID - jem.20010897 [pii]
AID - 20010897 [pii]
AID - 10.1084/jem.20010897 [doi]
PST - ppublish
SO  - J Exp Med. 2003 Aug 4;198(3):455-67. doi: 10.1084/jem.20010897.