PMID- 12900686
OWN - NLM
STAT- MEDLINE
DCOM- 20031211
LR  - 20131121
IS  - 0195-9131 (Print)
IS  - 0195-9131 (Linking)
VI  - 35
IP  - 8
DP  - 2003 Aug
TI  - Effect of prolonged exercise and carbohydrate on total neutrophil elastase 
      content.
PG  - 1326-32
AB  - PURPOSE: The purpose of the present study was twofold: first, to assess the 
      effect of prolonged intense exercise on total neutrophil elastase content in 
      endurance-trained cyclists and to determine whether this is associated with 
      postexercise falls in lipopolysaccharide (LPS)-stimulated neutrophil elastase 
      release; and second, to determine the effect of carbohydrate (CHO) ingestion 
      during exercise on these responses. METHODS: In a randomized design, nine trained 
      male cyclists cycled for 2 h at 75% VO(2max) on two occasions with either CHO 
      (6.4%, i.e., 64 g x L-1) or placebo (PLA) beverage ingestion before (5 mL x 
      kg-1), during (2 mL x kg-1), and after (5 mL x kg-1) the exercise. Venous blood 
      samples were obtained at rest, immediately postexercise, and at 1 h postexercise. 
      RESULTS: After exercise, CHO ingestion was associated with a higher plasma 
      glucose concentration (P < 0.05) and fewer numbers of circulating neutrophils 
      compared with the PLA trial (P < 0.01). Neither exercise nor CHO ingestion 
      affected total neutrophil elastase content, yet LPS-stimulated neutrophil 
      elastase release fell postexercise by approximately 47% on the PLA trial (P < 
      0.01). Values did not change significantly from preexercise on the CHO trial. 
      CONCLUSIONS: These findings suggest that neither exercise nor CHO-beverage 
      ingestion influences the total elastase content of neutrophils. Therefore, 
      changes in neutrophil elastase content cannot account for the fall in 
      LPS-stimulated neutrophil elastase release after prolonged intense exercise or 
      for the blunting of this response with CHO ingestion.
FAU - Bishop, Nicolette C
AU  - Bishop NC
AD  - School of Sport and Exercise Sciences, Loughborough University, Loughborough, 
      United Kingdom. N.C.Bishop@llboro.ac.uk
FAU - Walsh, Neil P
AU  - Walsh N
FAU - Scanlon, Gabriella A
AU  - Scanlon GA
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - United States
TA  - Med Sci Sports Exerc
JT  - Medicine and science in sports and exercise
JID - 8005433
RN  - 0 (Blood Glucose)
RN  - 0 (Carbohydrates)
RN  - 0 (Lipopolysaccharides)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Administration, Oral
MH  - Adult
MH  - Bicycling/*physiology
MH  - Blood Glucose/metabolism
MH  - Carbohydrates/*administration & dosage
MH  - Cell Degranulation/physiology
MH  - Exercise/*physiology
MH  - Heart Rate/physiology
MH  - Humans
MH  - Hydrocortisone/blood
MH  - Lactic Acid/blood
MH  - Leukocyte Count
MH  - Leukocyte Elastase/biosynthesis/*blood
MH  - Lipopolysaccharides/metabolism
MH  - Male
MH  - Neutrophils/*enzymology
MH  - Oxygen Consumption/physiology
MH  - Physical Endurance/*physiology
MH  - Physical Exertion/physiology
MH  - Plasma Volume/physiology
EDAT- 2003/08/06 05:00
MHDA- 2003/12/12 05:00
CRDT- 2003/08/06 05:00
PHST- 2003/08/06 05:00 [pubmed]
PHST- 2003/12/12 05:00 [medline]
PHST- 2003/08/06 05:00 [entrez]
AID - 10.1249/01.MSS.0000078927.08049.A8 [doi]
PST - ppublish
SO  - Med Sci Sports Exerc. 2003 Aug;35(8):1326-32. doi: 
      10.1249/01.MSS.0000078927.08049.A8.