PMID- 12903036
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20201208
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 20
IP  - 4
DP  - 2003 Aug
TI  - [Sequence analysis and expression study of BRI gene in two lung adenocarcinoma 
      cell lines with different metastatic potential].
PG  - 292-6
AB  - OBJECTIVE: To investigate the sequence of amyloid fibrils (BRI) gene and its 
      expression in two lung adenocarcinoma cell lines AGZY83-a and Anip973 with the 
      same tumor origin but different metastatic potential. METHODS: DNA sequencing, 
      sequential G banding fluorescence in situ hybridization (FISH) and Northern blot 
      were used to analyze the sequence and expression of BRI gene was in two lung 
      adenocarcinoma cell lines with different metastatic potential. RESULTS: The 
      expression of BRI gene was up-regulated in the highly metastatic cell line 
      Anip973 and was down-regulated in the low metastatic cell line AGZY83-a from 
      which the Anip973 was derived. FISH results disclosed that in the two cell lines, 
      the same rearrangements existed in the chromosome region where BRI gene was 
      located, but in Anip973, amplification took place in the chromosome region where 
      BRI gene was located. DNA sequencing results showed different mutations in the 5' 
      untranslated region of BRI gene in the two cell lines. CONCLUSION: The above 
      results revealed that there was no relation between BRI gene differential 
      expression and rearrangements of chromosome. The amplification of the chromosome 
      region where BRI gene was located and the different mutations in the 5' 
      untranslated region of BRI gene probably contributed to the differential 
      expression.
FAU - Yang, Huan-jie
AU  - Yang HJ
AD  - Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, 
      150086 PR China.
FAU - Li, Yu
AU  - Li Y
FAU - Chen, Ying-zhun
AU  - Chen YZ
FAU - Lu, Bing-jie
AU  - Lu BJ
FAU - Chen, Feng
AU  - Chen F
FAU - Chen, Yu
AU  - Chen Y
FAU - Li, Pu
AU  - Li P
FAU - Fu, Song-bin
AU  - Fu SB
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (ITM2B protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Adenocarcinoma/genetics/pathology
MH  - Blotting, Northern
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 13/genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/genetics/pathology
MH  - Membrane Glycoproteins
MH  - Membrane Proteins/*genetics
MH  - Neoplasm Metastasis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
EDAT- 2003/08/07 05:00
MHDA- 2009/03/04 09:00
CRDT- 2003/08/07 05:00
PHST- 2003/08/07 05:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2003/08/07 05:00 [entrez]
AID - 940602079 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Aug;20(4):292-6.