PMID- 12903046
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20091119
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 20
IP  - 4
DP  - 2003 Aug
TI  - [A dual-color fluorescence in situ hybridization study on the detection of 
      inv(16) in acute myeloid leukemia].
PG  - 331-5
AB  - OBJECTIVE: To explore the value of dual-color fluorescence in situ hybridization 
      (D-FISH) in the detection of inv(16) in acute myeloid leukemia (AML). METHODS: 
      Eleven AML patients were investigated by D-FISH with two-color break apart probe 
      for MYH11 labeled directly by fluorescein isocyanate (FITC) and a Texas Red. The 
      results were associated or compared with those of cell morphology, cytogenetics, 
      single color fluorescence in situ hybridization (FISH) and reverse 
      transcription-polymerase chain reaction (RT-PCR). RESULTS: Four cases (M4Eo three 
      cases, M2a one case) had inv(16), of which one had trisomy 22 in addition to 
      inv(16), while the other seven cases had no inv(16), of which, five cases (M4Eo 
      three cases, M4 two cases)had a normal karyotype, one (M2a) had 5p+ and trisomy 
      22, one (M4Eo) had a translocation t(9;22) on G-banded karyotypic analysis. All 
      11 cases of AML were positive for the rearrangement of inv(16) detected by 
      D-FISH. The average positive cell rate for these 11 AML patients was 93.45% 
      (range 86.6%-98.7%). Of them, four had a minimal deletion of 16p13 in addition to 
      inv(16). The results of D-FISH coincided with those of RT-PCR or single color 
      FISH. CONCLUSION: D-FISH is a powerful tool for the detection of inv(16) due to 
      its sensitivity and specificity. For raising the detecting rate of inv(16), it is 
      necessary to screen inv(16) rearrangement by D-FISH in all M4- and M2-AML cases 
      or the cases with trisomy 22, no matter whether they are accompanied by bone 
      marrow eosinophilia.
FAU - Li, Ming
AU  - Li M
AD  - First Affiliated Hospital, Soochow University, Jiangsu Institute of Haematology, 
      Suzhou, Jiangsu, 215006 PR China.
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Wu, Ya-fang
AU  - Wu YF
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Yao, Li
AU  - Yao L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - *Chromosome Inversion
MH  - Chromosomes, Human, Pair 16/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2003/08/07 05:00
MHDA- 2009/03/04 09:00
CRDT- 2003/08/07 05:00
PHST- 2003/08/07 05:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2003/08/07 05:00 [entrez]
AID - 940602089 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Aug;20(4):331-5.