PMID- 12904374
OWN - NLM
STAT- MEDLINE
DCOM- 20031023
LR  - 20220311
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 41
IP  - 8
DP  - 2003 Aug
TI  - Detection of circulating gp43 antigen in serum, cerebrospinal fluid, and 
      bronchoalveolar lavage fluid of patients with paracoccidioidomycosis.
PG  - 3675-80
AB  - Paracoccidioidomycosis (PCM) is an important systemic fungal disease, 
      particularly among individuals living and working in rural areas of endemicity in 
      Latin America, who, without antifungal therapy, may develop fatal acute or 
      chronic infection. For such patients, the detection of antibody responses by 
      immunodiffusion is of limited value due to false-negative results. In contrast, 
      the detection of Paracoccidioides brasiliensis gp43 circulating antigen may 
      represent a more practical approach to the rapid diagnosis of the disease. 
      Accordingly, an inhibition enzyme-linked immunosorbent assay (inh-ELISA) was 
      developed for the detection of a 43-kDa P. brasiliensis-specific epitope 
      incorporating a species-specific murine monoclonal antibody. With sera from 
      patients with acute and chronic forms of the disease (n = 81), the overall 
      sensitivity of the test was found to be 95.1%, while specificity was found to be 
      97.5% compared to that with normal human sera from blood donors (n = 93) and sera 
      from patients with other chronic fungal infections (histoplasmosis [n = 33] and 
      cryptococcosis [n = 20]). The inh-ELISA detected circulating antigen in 100% of 
      patients with the acute form of PCM and in 95.31 and 100% of patients with the 
      chronic multifocal and unifocal forms of PCM according to the patient's clinical 
      presentation. Cerebrospinal fluid from 14 patients with 
      neuroparacoccidioidomycosis and 13 samples of bronchoalveolar lavage fluid from 
      patients with pulmonary unifocal PCM were also tested for gp43 detection, with 
      the test showing 100% sensitivity and specificity. This novel, highly specific 
      inh-ELISA represents a significant addition to the existing tests for the 
      diagnosis of PCM.
FAU - Marques da Silva, Silvia Helena
AU  - Marques da Silva SH
AD  - Department of Microbiology, Immunology and Parasitology, Federal University of 
      Sao Paulo, Sao Paulo, Brazil.
FAU - Colombo, Arnaldo Lopes
AU  - Colombo AL
FAU - Blotta, Maria Heloisa Souza Lima
AU  - Blotta MH
FAU - Lopes, Jose Daniel
AU  - Lopes JD
FAU - Queiroz-Telles, Flavio
AU  - Queiroz-Telles F
FAU - Pires de Camargo, Zoilo
AU  - Pires de Camargo Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antibody Formation
MH  - Antigens, Fungal/*blood/cerebrospinal fluid
MH  - Brazil
MH  - Bronchoalveolar Lavage Fluid/*microbiology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Fungal Proteins/*blood/cerebrospinal fluid
MH  - Glycoproteins/*blood/cerebrospinal fluid
MH  - Humans
MH  - Paracoccidioides/isolation & purification
MH  - Paracoccidioidomycosis/blood/cerebrospinal fluid/*diagnosis/immunology
MH  - Reproducibility of Results
PMC - PMC179774
EDAT- 2003/08/09 05:00
MHDA- 2003/10/24 05:00
PMCR- 2003/08/01
CRDT- 2003/08/09 05:00
PHST- 2003/08/09 05:00 [pubmed]
PHST- 2003/10/24 05:00 [medline]
PHST- 2003/08/09 05:00 [entrez]
PHST- 2003/08/01 00:00 [pmc-release]
AID - 0063 [pii]
AID - 10.1128/JCM.41.8.3675-3680.2003 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2003 Aug;41(8):3675-80. doi: 10.1128/JCM.41.8.3675-3680.2003.